Design of microdevices for long-term live cell imaging

Chen, Huaying, Rosengarten, Gary, Li, Musen and Nordon, Robert E. (2012) Design of microdevices for long-term live cell imaging. Journal of Micromechanics and Microengineering, 22 6: 065033.1-065033.10. doi:10.1088/0960-1317/22/6/065033

Author Chen, Huaying
Rosengarten, Gary
Li, Musen
Nordon, Robert E.
Title Design of microdevices for long-term live cell imaging
Journal name Journal of Micromechanics and Microengineering   Check publisher's open access policy
ISSN 0960-1317
Publication date 2012-06
Year available 2012
Sub-type Article (original research)
DOI 10.1088/0960-1317/22/6/065033
Volume 22
Issue 6
Start page 065033.1
End page 065033.10
Total pages 11
Place of publication Bristol, United Kingdom
Publisher Institute of Physics Publishing
Collection year 2013
Language eng
Formatted abstract
Advances in fluorescent live cell imaging provide high-content information that relates a cell's life events to its ancestors. An important requirement to track clonal growth and development is the retention of motile cells derived from an ancestor within the same microscopic field of view for days to weeks, while recording fluorescence images and controlling the mechanical and biochemical microenvironments that regulate cell growth and differentiation. The aim of this study was to design a microwell device for long-term, time-lapse imaging of motile cells with the specific requirements of (a) inoculating devices with an average of one cell per well and (b) retaining progeny of cells within a single microscopic field of view for extended growth periods. A two-layer PDMS microwell culture device consisting of a parallel-plate flow cell bonded on top of a microwell array was developed for cell capture and clonal culture. Cell deposition statistics were related to microwell geometry (plate separation and well depth) and the Reynolds number. Computational fluid dynamics was used to simulate flow in the microdevices as well as cell–fluid interactions. Analysis of the forces acting upon a cell was used to predict cell docking zones, which were confirmed by experimental observations. Cell–fluid dynamic interactions are important considerations for design of microdevices for long-term, live cell imaging. The analysis of force and torque balance provides a reasonable approximation for cell displacement forces. It is computationally less intensive compared to simulation of cell trajectories, and can be applied to a wide range of microdevice geometries to predict the cell docking behavior.
Keyword Microfluidic Channels
Culture Array
Shear Stresses
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 18 Sep 2013, 10:36:22 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology