Functional characterization of hepatic transporters using intravital microscopy

Weiss, Michael, Liu, Xin, Thorling, Camilla A. and Roberts, Michael S. (2013) Functional characterization of hepatic transporters using intravital microscopy. European Journal of Pharmaceutical Sciences, 49 5: 845-849. doi:10.1016/j.ejps.2013.06.006

Author Weiss, Michael
Liu, Xin
Thorling, Camilla A.
Roberts, Michael S.
Title Functional characterization of hepatic transporters using intravital microscopy
Journal name European Journal of Pharmaceutical Sciences   Check publisher's open access policy
ISSN 0928-0987
Publication date 2013-08
Sub-type Article (original research)
DOI 10.1016/j.ejps.2013.06.006
Open Access Status
Volume 49
Issue 5
Start page 845
End page 849
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Abstract A better understanding of the role of hepatic transporters in drug elimination is of crucial importance for drug development and therapy. This study examined the usefulness of intravital microscopy to quantitatively evaluate the function of hepatic transporters in the exposed liver of anesthetized rats. In one experiment the function of the organic anion transporting polypeptide (Oatp) in sinusoidal uptake was investigated by administering an Oatp inhibitor, rifampicin, prior to the probe substrate Na-fluorescein. In another experiment, rhodamine 123 was used to quantify the biliary canalicular transporter P-glycoprotein (P-gp, Abcb1a/b) with cyclosporin A as an inhibitor of P-gp activity. Calibrated fluorescence intensity time curves measured in sinusoids and hepatocytes together with cumulative biliary excretion data from control and inhibitor treated animals were analyzed with a three-compartment model. A robust parameter estimation was achieved using nonlinear mixed effects modeling. Rifampicin reduced the hepatic uptake clearance of Na-fluorescein to 25% of the control (p < 0.05) without affecting other parameters. In the presence of cyclosporin A, biliary excretion of rhodamine 123 decreased to 7% of the control (p < 0.01). The novelty of this approach is that it allows a quantitative evaluation of transporter function in the in vivo rat liver.
Keyword Rat liver
Multiphoton tomography
Hepatobiliary disposition
Organic anion transporting polypeptide
Pharmacokinetic model
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
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