Hanging drop: an in vitro air toxic exposure model using human lung cells in 2D and 3D structures

Liu, Faye F., Peng, Cheng, Escher, Beate I., Fantino, Emmanuelle, Giles, Cindy, Were, Stephen, Duffy, Lesley and Ng, Jack C. (2013) Hanging drop: an in vitro air toxic exposure model using human lung cells in 2D and 3D structures. Journal of Hazardous Materials, 261 701-710. doi:10.1016/j.jhazmat.2013.01.027

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Liu, Faye F.
Peng, Cheng
Escher, Beate I.
Fantino, Emmanuelle
Giles, Cindy
Were, Stephen
Duffy, Lesley
Ng, Jack C.
Title Hanging drop: an in vitro air toxic exposure model using human lung cells in 2D and 3D structures
Formatted title
Hanging drop: an in vitro air toxic exposure model using human lung cells in 2D and 3D structures
Journal name Journal of Hazardous Materials   Check publisher's open access policy
ISSN 0304-3894
Publication date 2013-01-30
Sub-type Article (original research)
DOI 10.1016/j.jhazmat.2013.01.027
Volume 261
Start page 701
End page 710
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
Using benzene as a candidate air toxicant and A549 cells as an in vitro cell model, we have developed and validated a hanging drop (HD) air exposure system that mimics an air liquid interface exposure to the lung for periods of 1 h to over 20 days. Dose response curves were highly reproducible for 2D cultures but more variable for 3D cultures. By comparing the HD exposure method with other classically used air exposure systems, we found that the HD exposure method is more sensitive, more reliable and cheaper to run than medium diffusion methods and the CULTEX® system. The concentration causing 50% of reduction of cell viability (EC50) for benzene, toluene, p-xylene, m-xylene and o-xylene to A549 cells for 1 h exposure in the HD system were similar to previous in vitro static air exposure. Not only cell viability could be assessed but also sub lethal biological endpoints such as DNA damage and interleukin expressions. An advantage of the HD exposure system is that bioavailability and cell concentrations can be derived from published physicochemical properties using a four compartment mass balance model. The modelled cellular effect concentrations EC50cell for 1 h exposure were very similar for benzene, toluene and three xylenes and ranged from 5 to 15 mmol/kgdry weight, which corresponds to the intracellular concentration of narcotic chemicals in many aquatic species, confirming the high sensitivity of this exposure method.

Highlights ► Validated for the first time a hanging drop (HD) air toxic exposure method for inhalation exposures effect studies. ► Applied a four compartment mass balance model to calculate cellular exposure levels. ► Compared A549 2-dimentional (2D) cell culture and 3D cell cultures benzene sensitivity. ► Carried out up to 20 days in vitro exposure studies. ► Validated A549 cell hanging drop toluene and xylene exposure EC50 value with published static apical air exposure method.
Keyword Hanging drop
In vitro air exposure
Lung cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 30 January 2013

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 11 Sep 2013, 21:10:32 EST by Dr Cheng Peng on behalf of National Res Centre For Environmental Toxicology