Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase enzymes for hFSH produced by recombinant CHO cells

Chotigeat, W., Watanapokasin, Y., Mahler, S. M. and Gray, P. P. (1994) Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase enzymes for hFSH produced by recombinant CHO cells. Cytotechnology, 15 1-3: 217-221. doi:10.1007/BF00762396


Author Chotigeat, W.
Watanapokasin, Y.
Mahler, S. M.
Gray, P. P.
Title Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase enzymes for hFSH produced by recombinant CHO cells
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
1573-0778
Publication date 1994-02-01
Sub-type Article (original research)
DOI 10.1007/BF00762396
Volume 15
Issue 1-3
Start page 217
End page 221
Total pages 5
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Formatted abstract
A recombinant CHO cell line in which the expresison of human follicle stimulating hormone (hFSH) was under the control of the β actin promoter was maintained in steady state perfusion cultures on a protein free medium. The level of expression of the hFSH was controlled by varying the steady state level of dissolved oxygen (10–90% of air saturation) and of sodium butyrate (0–1.5mM). Under these conditions, the specific productivity of hFSH (qFSH) varied from 0.7 to 4.8 ng hFSH/106 cells/h. As the specific productivity of hFSH increased, there was a shift in the FSH isoforms to the lower pI fractions, corresponding to increased sialic acid content. As the specific productivity of hFSH increased, shifting the isoform distribution towards the lower pI isoforms, that the sialyltransferase enzymic activity also increased.
Keyword Beta actin
CHO
hFSH
Perfusion cultures
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 09 Sep 2013, 14:46:01 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology