Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line

Davis, Felicity M., Parsonage, Michelle T., Cabot, Peter J., Parat, Marie-Odile, Thompson, Erik W., Roberts-Thomson, Sarah J. and Monteith, Gregory R. (2013) Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line. Cancer Cell International, 13 76: 1-7. doi:10.1186/1475-2867-13-76


Author Davis, Felicity M.
Parsonage, Michelle T.
Cabot, Peter J.
Parat, Marie-Odile
Thompson, Erik W.
Roberts-Thomson, Sarah J.
Monteith, Gregory R.
Title Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line
Journal name Cancer Cell International   Check publisher's open access policy
ISSN 1475-2867
Publication date 2013-07
Sub-type Article (original research)
DOI 10.1186/1475-2867-13-76
Open Access Status DOI
Volume 13
Issue 76
Start page 1
End page 7
Total pages 7
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2014
Language eng
Formatted abstract
Background Epithelial-mesenchymal transition (EMT) is a process implicated in cancer metastasis that involves the conversion of epithelial cells to a more mesenchymal and invasive cell phenotype. In breast cancer cells EMT is associated with altered store-operated calcium influx and changes in calcium signalling mediated by activation of cell surface purinergic receptors. In this study, we investigated whether MDA-MB-468 breast cancer cells induced to undergo EMT exhibit changes in mRNA levels of calcium channels, pumps and exchangers located on intracellular calcium storing organelles, including the Golgi, mitochondria and endoplasmic reticulum (ER).

Methods Epidermal growth factor (EGF) was used to induce EMT in MDA-MB-468 breast cancer cells. Serum-deprived cells were treated with EGF (50 ng/mL) for 12 h and gene expression was assessed using quantitative RT-PCR.

Results and conclusions These data reveal no significant alterations in mRNA levels of the Golgi calcium pump secretory pathway calcium ATPases (SPCA1 and SPCA2), or the mitochondrial calcium uniporter (MCU) or Na+/Ca2+ exchanger (NCLX). However, EGF-induced EMT was associated with significant alterations in mRNA levels of specific ER calcium channels and pumps, including (sarco)-endoplasmic reticulum calcium ATPases (SERCAs), and inositol 1,4,5-trisphosphate receptor (IP3R) and ryanodine receptor (RYR) calcium channel isoforms. The most prominent change in gene expression between the epithelial and mesenchymal-like states was RYR2, which was enriched 45-fold in EGF-treated MDA-MB-468 cells. These findings indicate that EGF-induced EMT in breast cancer cells may be associated with major alterations in ER calcium homeostasis.
Keyword Breast cancer calcium
EMT
IP3R
RYR
SERCA
SPCA
MCU
NCLX
Down-regulation
Differential expression
Endoplasmic-reticulum
Essential component
Confers resistance
Mitochondrial Ca2+
Release channels
Smooth-muscle
Up-regulation
Uniporter
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733826/

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Pharmacy Publications
 
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