Molecular analysis of the Acinetobacter baumannii biofilm-associated protein

Goh, H. M. Sharon, Beatson, Scott A., Totsika, Makrina, Moriel, Danilo G., Phan, Minh-Duy, Szubert, Jan, Runnegar, Naomi, Sidjabat, Hanna E., Paterson, David L., Nimmo, Graeme R., Lipman, Jeffrey and Schembri, Mark A. (2013) Molecular analysis of the Acinetobacter baumannii biofilm-associated protein. Applied and Environmental Microbiology, 79 21: 6535-6543. doi:10.1128/AEM.01402-13

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Author Goh, H. M. Sharon
Beatson, Scott A.
Totsika, Makrina
Moriel, Danilo G.
Phan, Minh-Duy
Szubert, Jan
Runnegar, Naomi
Sidjabat, Hanna E.
Paterson, David L.
Nimmo, Graeme R.
Lipman, Jeffrey
Schembri, Mark A.
Title Molecular analysis of the Acinetobacter baumannii biofilm-associated protein
Formatted title
Molecular analysis of the Acinetobacter baumannii biofilm-associated protein
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2013-11
Sub-type Article (original research)
DOI 10.1128/AEM.01402-13
Open Access Status File (Publisher version)
Volume 79
Issue 21
Start page 6535
End page 6543
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2014
Language eng
Formatted abstract
Acinetobacter baumannii is a multidrug resistant pathogen associated with hospital outbreaks of infection across the globe, particularly in the intensive care unit. The ability of A. baumannii to survive in the hospital environment for long periods is linked to antibiotic resistance and its capacity to form biofilms. Here we studied the prevalence, expression and function of the A. baumannii biofilm-associated protein (Bap) in 24 carbapenem-resistant A. baumannii ST92 strains isolated from a single institution over a 10-year period. The bap gene was highly prevalent, with 22/24 strains positive for bap by PCR. Partial sequencing of bap was performed on the index case strain MS1968 and revealed it to be a large and highly repetitive gene approximately 16 kb in size. Phylogenetic analysis employing a 1,948 amino acid region corresponding to the C-terminus of Bap showed BapMS1968 clusters with Bap sequences from clonal complex (CC) 2 strains ACICU, TCDC-AB0715 and 1656-2, and is distinct from CC1 strains. Using overlapping PCR, the bapMS1968 gene was cloned and its expression in a recombinant E. coli strain resulted in increased biofilm formation. A Bap-specific antibody was generated and western blot analysis showed the majority of A. baumannii strains expressed an ~200 kDa Bap protein. Further analysis of three Bap-positive A. baumannii strains demonstrated Bap is expressed at the cell surface and is associated with biofilm formation. Finally, biofilm formation by these Bap-positive strains could be inhibited by affinity-purified Bap antibodies, demonstrating the direct contribution of Bap to biofilm growth by A. baumannii clinical isolates.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published ahead of print: 16 August 2013.

 
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Created: Mon, 02 Sep 2013, 15:06:17 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences