Isolation and diversity of planctomycetes from the sponge Niphates sp., seawater, and sediment of Moreton Bay, Australia.

Izumi, Hiroshi, Sagulenko, Evgeny, Webb, Richard I. and Fuerst, John A. (2013) Isolation and diversity of planctomycetes from the sponge Niphates sp., seawater, and sediment of Moreton Bay, Australia.. Antonie Van Leeuwenhoek, 104 4: 533-546. doi:10.1007/s10482-013-0003-5

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Author Izumi, Hiroshi
Sagulenko, Evgeny
Webb, Richard I.
Fuerst, John A.
Title Isolation and diversity of planctomycetes from the sponge Niphates sp., seawater, and sediment of Moreton Bay, Australia.
Formatted title
Isolation and diversity of planctomycetes from the sponge Niphates sp., seawater, and sediment of Moreton Bay, Australia.
Journal name Antonie Van Leeuwenhoek   Check publisher's open access policy
ISSN 0003-6072
1572-9699
Publication date 2013-10
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s10482-013-0003-5
Volume 104
Issue 4
Start page 533
End page 546
Total pages 14
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2014
Language eng
Formatted abstract
Planctomycetes are ubiquitous in marine environment and were reported to occur in association with multicellular eukaryotic organisms such as marine macroalgae and invertebrates. Here, we investigate planctomycetes associated with the marine sponge Niphates sp. from the sub-tropical Australian coast by assessing their diversity using culture-dependent and -independent approaches based on the 16S rRNA gene. The culture-dependent approach resulted in the isolation of a large collection of diverse planctomycetes including some novel lineages of Planctomycetes from the sponge as well as sediment and seawater of Moreton Bay where this sponge occurs. The characterization of these novel planctomycetes revealed that cells of one unique strain do not possess condensed nucleoids, a phenotype distinct from other planctomycetes. In addition, a culture-independent clone library approach identified unique planctomycete 16S rRNA gene sequences closely related to other sponge-derived sequences. The analysis of tissue of the sponge Niphates sp. showed that the mesohyl of the sponge is almost devoid of microbial cells, indicating this species is in the group of ‘low microbial abundant’ (LMA) sponges. The unique planctomycete 16S rRNA gene sequences identified in this study were phylogenetically closely related to sequences from LMA sponges in other published studies. This study has revealed new insights into the diversity of planctomycetes in the marine environment and the association of planctomycetes with marine sponges.
Keyword Planctomycetes
Marine sponges
16S rRNA gene
Culture-dependent
Culture-independent
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 20 August 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
Centre for Microscopy and Microanalysis Publications
 
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Created: Mon, 02 Sep 2013, 15:03:52 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences