Changes in the redox state and endogenous fluorescence of in vivo human skin due to intrinsic and photo-aging, measured by multiphoton tomography with fluorescence lifetime imaging

Sanchez, Washington Y., Obispo, Clara, Ryan, Elizabeth, Grice, Jeffrey E. and Roberts, Michael S. (2013) Changes in the redox state and endogenous fluorescence of in vivo human skin due to intrinsic and photo-aging, measured by multiphoton tomography with fluorescence lifetime imaging. Journal of Biomedical Optics, 18 6: 61217.1-61217.12. doi:10.1117/1.JBO.18.6.061217

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ308097_OA.pdf Full text (open access) application/pdf 4.79MB 0

Author Sanchez, Washington Y.
Obispo, Clara
Ryan, Elizabeth
Grice, Jeffrey E.
Roberts, Michael S.
Title Changes in the redox state and endogenous fluorescence of in vivo human skin due to intrinsic and photo-aging, measured by multiphoton tomography with fluorescence lifetime imaging
Journal name Journal of Biomedical Optics   Check publisher's open access policy
ISSN 1083-3668
1560-2281
Publication date 2013-06
Year available 2012
Sub-type Article (original research)
DOI 10.1117/1.JBO.18.6.061217
Open Access Status File (Publisher version)
Volume 18
Issue 6
Start page 61217.1
End page 61217.12
Total pages 12
Place of publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Collection year 2014
Language eng
Abstract Ultraviolet radiation from solar exposure is a key extrinsic factor responsible for premature skin aging (i.e., photo-aging). Recent advances using in vivo multiphoton tomography (MPT) demonstrate the efficacy of this approach to assess intrinsic and extrinsic skin aging as an alternative to existing invasive techniques. In this study, we measured changes in epidermal autofluorescence, dermal collagen second harmonic generation (SHG), and the redox state of solar-exposed and solar-protected human skin by MPT with fluorescence lifetime imaging (MPT-FLIM). Twenty-four volunteers across four age categories (20 to 29, 30 to 39, 40 to 49, and 50 to 59 years old; six volunteers each) were recruited for MPT-FLIM imaging of the dorsal (solar-exposed; photo-damaged) and volar (solar-protected) forearm. We demonstrate a higher intensity of dermal collagen SHG within the volar forearm compared to dorsal solar-exposed skin. Redox imaging of each epidermal skin stratum by FLIM demonstrates an increase in fluorescence lifetime in the solar-exposed dorsal forearm that is more apparent in aged skin. The results of this study suggest the redox state of the viable epidermis is a key marker in assessing intrinsic and photo-damage skin aging, in combination with changes in autofluorescence and SHG.
Keyword Photo-aging
Skin
Redox
Fluorescence lifetime imaging
Photo-damage
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 01 Sep 2013, 00:12:07 EST by System User on behalf of School of Medicine