Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia

AbdulMajeed, Ahmad A., Dalley, Andrew J. and Farah, Camile S. (2013) Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia. Annals of Diagnostic Pathology, 17 4: 331-340. doi:10.1016/j.anndiagpath.2013.03.003


Author AbdulMajeed, Ahmad A.
Dalley, Andrew J.
Farah, Camile S.
Title Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia
Journal name Annals of Diagnostic Pathology   Check publisher's open access policy
ISSN 1092-9134
1532-8198
Publication date 2013-08
Sub-type Article (original research)
DOI 10.1016/j.anndiagpath.2013.03.003
Volume 17
Issue 4
Start page 331
End page 340
Total pages 10
Place of publication Maryland Heights, MO, United States
Publisher W.B. Saunders
Collection year 2014
Language eng
Formatted abstract
Early diagnosis and targeted therapy are crucial to mitigating the morbidity and mortality of oral squamous cell carcinoma. Among the potentially malignant oral disorders, epithelial dysplasia has known association with malignant transformation, but defensible gradation of dysplasia severity presents unmet challenges. Published microarray data has denoted dysregulation of CLSP, ELF3, IFI44, USP18, and CXCL13 genes in potentially malignant oral disorders. The present study investigated the diagnostic potential of these gene products to grade oral epithelial dysplasia severity. Archived biopsies from independent patient cohorts comprised “training” (n = 107) and “test” (n = 278) sample sets. Immunoreactivity for candidate markers was determined in the “training” set of normal oral mucosa (NOM), mild dysplasia (MD), moderate to severe dysplasia, and oral squamous cell carcinoma (OSCC). The diagnostic potential of ELF3 immunoscoring to improve detection and severity gradation of epithelial dysplasia was assessed with the “test” set. A reciprocal relationship between disease severity and immunoreactivity score for CLSP and ELF3 was observed (MD/NOM to OSCC: P < .08, Mann-Whitney U test), whereas elevated IFI44 immunostaining was present for OSCC compared to MD/NOM (P < .08, Mann-Whitney U test). Loss of ELF3 immunostaining effectively distinguished OSCC from non-malignant tissues (sensitivity = 0.81; specificity = 0.56; area under the curve [AUC] = 0.68) but did not distinguish dysplasia from NOM (sensitivity = 0.55; specificity = 0.40; AUC = 0.47) or moderate to severe dysplasia from MD (sensitivity = 0.63; specificity = 0.51; AUC = 0.57). The results confirm via immunohistochemistry the relevance of published CLSP, ELF3, and IFI44 (but not USP18 or CXCL13) gene expression data to potentially malignant oral lesion severity. Loss of ELF3 immunostaining discriminated OSCC from dysplasia but was unreliable for grading dysplasia severity.
Keyword Oral epithelial dysplasia
Oral squamous cell carcinoma
Organotypic culture
Immunohistochemistry
ELF3
CLSP
Gene-expression signatures
Mesenchymal transition
Oligonucleotide microarray
Neck-cancer
Identification
Leukoplakia
Biomarkers
Invasion
Protein
Lesions
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Dentistry Publications
 
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