Functionalised polycaprolactone films and 3D scaffolds via gamma irradiation-induced grafting

Luk, Jing Zhong, Cooper-White, Justin, Rintoul, Llew, Taran, Elena and Grondahl, Lisbeth (2013) Functionalised polycaprolactone films and 3D scaffolds via gamma irradiation-induced grafting. Journal of Materials Chemistry B, 1 33: 4171-4181. doi:10.1039/c3tb20267d


Author Luk, Jing Zhong
Cooper-White, Justin
Rintoul, Llew
Taran, Elena
Grondahl, Lisbeth
Title Functionalised polycaprolactone films and 3D scaffolds via gamma irradiation-induced grafting
Journal name Journal of Materials Chemistry B   Check publisher's open access policy
ISSN 2050-750X
2050-7518
Publication date 2013-01
Year available 2013
Sub-type Article (original research)
DOI 10.1039/c3tb20267d
Open Access Status Not Open Access
Volume 1
Issue 33
Start page 4171
End page 4181
Total pages 11
Place of publication Cambridge, United Kingdom
Publisher R S C Publications
Collection year 2014
Language eng
Formatted abstract
Polycaprolactone (PCL) is frequently used as the base polymer in scaffolds targeted for tissue engineering applications. However, in the absence of further surface modification, the lack of functional moieties on the PCL chain results in non-ideal surface properties of such scaffolds, especially in terms of the inability to tailor the presentation of functional ligands for directed cell adhesion and growth. The current study investigates gamma irradiation-induced grafting as a means of improving the biofunctionality of the PCL surface. The surface presentation of carboxylic acid groups on 2D PCL films could be tailored by changing the acrylic acid (AAc) concentration and/or the solvent during grafting, as evaluated from X-ray photoelectron spectroscopy (XPS). From data obtained using Raman spectroscopy, it was concluded that the penetration depth of the grafted pAAc was affected by the solvent system with a mixed water-methanol system yielding high penetration. Grafted samples displayed a decreased elastic modulus of the surface correlating with pAAc penetration depth, as shown by nano-indentation using atomic force microscopy (AFM). The most promising grafting conditions found for the 2D PCL films were then applied to 3D thermally induced phase separation (TIPS) scaffolds and it was demonstrated using XPS that equivalent levels of grafting of pAAc could be achieved throughout the whole depth of the scaffold. The scaffolds maintained their overall integrity after grafting, even though we observed a decrease in the compressive modulus by 20% after surface modification. These combined studies confirm the utility of this surface modification methodology for scaffolds targeted at tissue engineering and cell culture applications.
Keyword Acrylic Acid
Surface Modification
Mechanical Properties
Methacrylic acid
Radiation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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