Visualization of intracellular hydrogen peroxide with HyPer, a genetically encoded fluorescent probe

Mishina, Nataliya M., Markvicheva, Kseniya N., Bilan, Dmitry S., Matlashov, Mikhail E., Shirmanova, Marina V., Liebl, David, Schultz, Carsten, Lukyanov, Sergey and Belousov, Vsevolod V. (2013) Visualization of intracellular hydrogen peroxide with HyPer, a genetically encoded fluorescent probe. Hydrogen Peroxide and Cell Signaling, Part a, 526 45-59. doi:10.1016/B978-0-12-405883-5.00003-X


Author Mishina, Nataliya M.
Markvicheva, Kseniya N.
Bilan, Dmitry S.
Matlashov, Mikhail E.
Shirmanova, Marina V.
Liebl, David
Schultz, Carsten
Lukyanov, Sergey
Belousov, Vsevolod V.
Title Visualization of intracellular hydrogen peroxide with HyPer, a genetically encoded fluorescent probe
Journal name Hydrogen Peroxide and Cell Signaling, Part a   Check publisher's open access policy
ISSN 0076-6879
1557-7988
ISBN 9780124058835
Publication date 2013-01
Year available 2013
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/B978-0-12-405883-5.00003-X
Open Access Status
Volume 526
Start page 45
End page 59
Total pages 15
Editor Enrique Cadenas
Lester Packer
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2014
Language eng
Formatted abstract
The fluorescent sensor HyPer allows monitoring of intracellular H2O2 levels with a high degree of sensitivity and specificity. Here, we provide a detailed protocol of ratiometric imaging of H2O2 produced by cells during phagocytosis, including instructions for experiments on different commercial confocal systems, namely, Leica SP2, Leica SP5, and Carl Zeiss LSM, as well as wide-field Leica 6000 microscope. The general experimental scheme is easily adaptable for imaging H2O2 production by various cell types under a variety of conditions.
Keyword Hydrogen peroxide
Reactive oxygen species
Sensor
HyPer
Imaging
Phagocytosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Special issue: Hydrogen Peroxide and Cell Signaling, Part A. Chapter 3.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
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