Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice

Aisha, Abdalrahim Fa, Ismail, Zhari, Abu-Salah, Khalid M., Siddiqui, Jamshed M., Ghafar, Gheniya and Abdul Majid, Amin Malik Shah (2013) Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice. BMC Complementary and Alternative Medicine, 13 168.1-168.11. doi:10.1186/1472-6882-13-168


Author Aisha, Abdalrahim Fa
Ismail, Zhari
Abu-Salah, Khalid M.
Siddiqui, Jamshed M.
Ghafar, Gheniya
Abdul Majid, Amin Malik Shah
Title Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
Journal name BMC Complementary and Alternative Medicine   Check publisher's open access policy
ISSN 1472-6882
Publication date 2013-07
Sub-type Article (original research)
DOI 10.1186/1472-6882-13-168
Open Access Status DOI
Volume 13
Start page 168.1
End page 168.11
Total pages 11
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2014
Language eng
Formatted abstract
Background: Syzygium campanulatum Korth (Myrtaceae) is an evergreen shrub rich in phenolics, flavonoid antioxidants, and betulinic acid. This study sought to investigate antiangiogenic and anti-colon cancer effects of S.C. standardized methanolic extract.

Methods: Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LC-MS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice.

Results: Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml). S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death.

Conclusion: Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid.
Keyword In-Vitro
Antiangiogenic Activity
Betulinic Acid
Therapeutic Implications
Cell-Migration
Cancer-Cells
Assay
Plants
Adenocarcinoma
Expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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