Accurate detection of methylated cytosine in complex methylation landscapes

Palanisamy, Ramkumar, Connolly, Ashley R. and Trau, Matt (2013) Accurate detection of methylated cytosine in complex methylation landscapes. Analytical Chemistry, 85 14: 6575-6579. doi:10.1021/ac3031948


Author Palanisamy, Ramkumar
Connolly, Ashley R.
Trau, Matt
Title Accurate detection of methylated cytosine in complex methylation landscapes
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
1520-6882
Publication date 2013-07
Sub-type Article (original research)
DOI 10.1021/ac3031948
Volume 85
Issue 14
Start page 6575
End page 6579
Total pages 5
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2014
Language eng
Abstract Monitoring DNA methylation can be a useful biomarker for disease diagnosis and prognosis. However, monitoring the methylation status of a specific cytosine biomarker is often confounded by heterogeneous peripheral DNA methylation. To address this issue, molecular inversion probes were designed with inosine strategically positioned to complement suspected DNA methylation sites. This enabled the methylation status of a specific cytosine to be accurately measured with a high level of specificity, irrespective of adjacent epigenetic modifications.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 01 Sep 2013, 00:07:11 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology