Analysis of promoter sequences from Lactobacillus and Lactococcus and their activity in several Lactobacillus species

McCracken, Andrea, Turner, Mark S., Giffard, Phil, Hafner, Louise M. and Timms, Peter (2000) Analysis of promoter sequences from Lactobacillus and Lactococcus and their activity in several Lactobacillus species. Archives of Microbiology, 173 5-6: 383-389. doi:10.1007/s002030000159


Author McCracken, Andrea
Turner, Mark S.
Giffard, Phil
Hafner, Louise M.
Timms, Peter
Title Analysis of promoter sequences from Lactobacillus and Lactococcus and their activity in several Lactobacillus species
Formatted title
Analysis of promoter sequences from Lactobacillus and Lactococcus and their activity in several Lactobacillus species
Journal name Archives of Microbiology   Check publisher's open access policy
ISSN 0302-8933
1432-072X
Publication date 2000-05
Sub-type Article (original research)
DOI 10.1007/s002030000159
Open Access Status
Volume 173
Issue 5-6
Start page 383
End page 389
Total pages 7
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Promoter-active fragments were isolated from the genome of the probiotic organism Lactobacillus rhamnosus strain GG using the promoter-probe vector pNZ272. These promoter elements, together with a promoter fragment isolated from the vaginal strain Lactobacillus fermentum BR11 and two previously defined promoters (Lactococcus lactis lacA and Lactobacillus acidophilus ATCC 4356 slpA), were introduced into three strains of Lactobacillus. Primer-extension analysis was used to map the transcriptional start site for each promoter. All promoter fragments tested were functional in each of the three lactobacilli and a purine residue was used to initiate transcription in most cases. The promoter elements encompassed a 52- to 1140-fold range in promoter activity depending on the host strain. Lactobacillus promoters were further examined by surveying previously mapped sequences for conserved base positions. The Lactobacillus hexamer regions (–35: TTgaca and –10: TAtAAT) closely resembled those of Escherichia coli and Bacillus subtilis, with the highest degree of agreement at the –10 hexamer. The TG dinucleotide upstream of the –10 hexamer was conserved in 26% of Lactobacillus promoters studied, but conservation rates differed between species. The region upstream of the –35 hexamer of Lactobacillus promoters showed conservation with the bacterial UP element.
Keyword Lactobacillus
promoter
consensus sequence
gene expression
Layer Protein Gene
Escherichia-Coli
Bacterial Promoters
Rna-Polymerase
Nucleotide-Sequence
Bacillus-Subtilis
Messenger-Rna
Expression
Identification
Cloning
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Wed, 28 Aug 2013, 20:57:39 EST by Dr Mark Turner on behalf of School of Agriculture and Food Sciences