DNA shuffling of cytochrome P450 enzymes

Behrendorff, James B. Y. H., Johnston, Wayne A. and Gillam, Elizabeth M. J. (2013). DNA shuffling of cytochrome P450 enzymes. In Ian R. Phillips, Elizabeth A. Shepherd and Paul R. Ortiz de Montellano (Ed.), Cytochrome P450 protocols 3rd ed. (pp. 177-188) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-321-3_16

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Author Behrendorff, James B. Y. H.
Johnston, Wayne A.
Gillam, Elizabeth M. J.
Title of chapter DNA shuffling of cytochrome P450 enzymes
Title of book Cytochrome P450 protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-62703-321-3_16
Year available 2013
Series Methods in Molecular Biology
Edition 3rd
ISBN 9781627033206
9781627033213
ISSN 1064-3745
1940-6029
Editor Ian R. Phillips
Elizabeth A. Shepherd
Paul R. Ortiz de Montellano
Volume number 987
Chapter number 16
Start page 177
End page 188
Total pages 12
Total chapters 25
Collection year 2014
Language eng
Abstract/Summary DNA family shuffling is an efficient method for creating libraries of novel enzymes, in which a high proportion of mutants exhibit correct folding and possess catalytic properties distinct from the starting material. The evolutionary arrangement of cytochromes P450 into subfamilies of enzymes with highly similar nucleotide sequences but distinct catalytic properties renders them excellent starting material for DNA family shuffling experiments. This chapter provides a general method for creating libraries of shuffled P450s from two or more related sequences and incorporates several recent improvements to previously published methods.
Keyword Cytochrome P450
DNA shuffling
Directed evolution
Biocatalysts
Mutant libraries
Protein engineering
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 27 Aug 2013, 15:02:06 EST by Anthony Yeates on behalf of Examinations