Partial separation of individual enzyme activities of an ACP-dependent fatty acid synthetase from barley chloroplasts

Høj, Peter Bordier and Mikkelsen, Jorn Dalgaard (1982) Partial separation of individual enzyme activities of an ACP-dependent fatty acid synthetase from barley chloroplasts. Carlsberg Research Communications, 47 2: 119-141. doi:10.1007/BF02914031


Author Høj, Peter Bordier
Mikkelsen, Jorn Dalgaard
Title Partial separation of individual enzyme activities of an ACP-dependent fatty acid synthetase from barley chloroplasts
Journal name Carlsberg Research Communications   Check publisher's open access policy
ISSN 0105-1938
Publication date 1982-01
Sub-type Article (original research)
DOI 10.1007/BF02914031
Volume 47
Issue 2
Start page 119
End page 141
Total pages 23
Place of publication Copenhagen, Denmark
Publisher Carlsberg Laboratoriet
Language eng
Abstract An acyl carrier protein (ACP) dependent fatty acid synthetase (fas) from barley chloroplast stroma was purified five-fold by ammonium sulphate precipitation and gel filtration on Sephacryl S-300. The β-ketoacyl-ACP reductase, β-ketoacyl-ACP synthetase, acetyl-CoA:ACP transacylase and malonyl-CoA:ACP transacylase activities were resolved on the Sephacryl S-300 column with apparent molecular weights of respectively 125, 92, 82 and 41 kilodalton. The fas activity exhibited an apparent molecular weight of 87 kilodalton resulting from the overlapping portions of the component activities. A fifth component of the active fas, ACP, was separated completely from the other four individual enzyme activities by the ammonium sulphate precipitation. When the fas purified by gel filtration was applied to a Mātrex Gel Blue B column, the component activities were separated into two groups. A bound fraction contained all the malonyl-CoA:ACP transacylase whereas the β-ketoacyl synthetase activity was exclusively present in the non-bound fraction. Neither the bound nor the non-bound fraction showed any fas activity alone, but complete reconstitution of fas activity was obtained when both protein fractions were combined. The barley chloroplast fas is therefore not a multifunctional protein but consists of at least five separable components. Characterization with respect to cofactor requirements was also performed. Variation of certain cofactor concentrations markedly altered the pattern of fatty acid synthesis.
Keyword Affinity chromatography
Arsenite
Coenzyme A derivatives
Fatty acids
Gel filtration
Monofunctional polypeptide
Multienzyme complex
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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