Purification and characterization of (1-]3, 1-]4)-beta-gluca endohydrolases from germinated wheat (Triticum aestivum)

Lai, Doreen M.L., Høj, Peter B. and Fincher, Geoffrey B. (1993) Purification and characterization of (1-]3, 1-]4)-beta-gluca endohydrolases from germinated wheat (Triticum aestivum). Plant Molecular Biology, 22 5: 847-859. doi:10.1007/BF00027370


Author Lai, Doreen M.L.
Høj, Peter B.
Fincher, Geoffrey B.
Title Purification and characterization of (1-]3, 1-]4)-beta-gluca endohydrolases from germinated wheat (Triticum aestivum)
Formatted title
Purification and characterization of (1→3, 1→4)-β-gluca endohydrolases from germinated wheat (Triticum aestivum)
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
1573-5028
Publication date 1993-08
Sub-type Article (original research)
DOI 10.1007/BF00027370
Volume 22
Issue 5
Start page 847
End page 859
Total pages 13
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Abstract A (1→3, 1→4)-β-glucan 4-glucanohydrolase [(1→3, 1→4)-β-glucanase, EC 3.2.1.73] was purified to homogeneity from extracts of germinated wheat grain. The enzyme, which was identified as an endohydrolase on the basis of oligosaccharide products released from a (1→3, 1→4)-β-glucan substrate, has an apparent pI of 8.2 and an apparent molecular mass of 30 kDa. Western blot analyses with specific monoclonal antibodies indicated that the enzyme is related to (1→3, 1→4)-β-glucanase isoenzyme EI from barley. The complete primary structure of the wheat (1→3, 1→4)-β-glucanase has been deduced from nucleotide sequence analysis of cDNAs isolated from a library prepared using poly(A)+ RNA from gibberellic acid-treated wheat aleurone layers. One cDNA, designated λLW2, is 1426 nucleotide pairs in length and encodes a 306 amino acid enzyme, together with a NH2-terminal signal peptide of 28 amino acid residues. The mature polypeptide encoded by this cDNA has a molecular mass of 32085 and a predicted pI of 8.1. The other cDNA, designated λLW1, carries a 109 nucleotide pair sequence at its 5′ end that is characteristic of plant introns and therefore appears to have been synthesized from an incompletely processed mRNA. Comparison of the coding and 3′-untranslated regions of the two cDNAs reveals 31 nucleotide substitutions, but none of these result in amino acid substitutions. Thus, the cDNAs encode enzymes with identical primary structures, but their corresponding mRNAs may have originated from homeologous chromosomes in the hexaploid wheat genome.
Keyword Amino acid sequence
cDNA
Germination
(1-]3, 1-]4)-beta-glucanases
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 26 Aug 2013, 17:18:35 EST by System User on behalf of Office of the Vice-Chancellor