A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution

Moller, Birger Lindberg and Hoj, Peter Bordier (1983) A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution. Carlsberg Research Communications, 48 3: 161-185. doi:10.1007/BF02907765


Author Moller, Birger Lindberg
Hoj, Peter Bordier
Title A thylakoid polypeptide involved in the reconstitution of photosynthetic oxygen evolution
Journal name Carlsberg Research Communications   Check publisher's open access policy
ISSN 0105-1938
Publication date 1983-01
Sub-type Article (original research)
DOI 10.1007/BF02907765
Volume 48
Issue 3
Start page 161
End page 185
Total pages 25
Place of publication Copenhagen, Denmark
Publisher Carlsberg Laboratoriet
Language eng
Abstract Sonication of barley thylakoids in a high salt buffer released three polypeptides of Mr 32,000, 23,000, and 13,500 which were purified to homogeneity by chromatofocusing. Highly purified inside-out photosystem II preparations were obtained by French Press treatment or by Triton X-100 fractionation of stacked lamellar systems. Both preparations are composed of pairs of appressed membrane sheets. In the French Press preparation, the majority of these membrane pairs are sealed whereas they are predominantly unsealed in the Triton X-100 preparation. Both preparations are able to evolve oxygen and show reversed proton pumping. The oxygen evolving capacity and variable fluorescence of both preparations were lost upon washing with high salt buffer. This treatment also removed three polypeptides at Mr 32,000, 23,000, and 13,500. The inactivated preparations were reconstituted with respect to oxygen evolution and variable fluorescence by rebinding of the isolated Mr 23,000 polypeptide. The Mr 32,000 and 13,500 polypeptides had no effect on reconstitution.
Keyword Barley
Chromatofocusing
Electron microscopy
Histones
Hydroxylamine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 33 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 26 Aug 2013, 17:17:54 EST by System User on behalf of Office of the Vice-Chancellor