Use of electrospray mass spectrometry for mass determination of grape (Vitis vinifera) juice pathogenesis-related proteins: a potential tool for varietal differentiation

Hayasaka, Yoji, Adams, Kathryn S., Pocock, Kenneth F., Baldock, Gayle A., Waters, Elizabeth J. and Hoj, Peter B. (2001) Use of electrospray mass spectrometry for mass determination of grape (Vitis vinifera) juice pathogenesis-related proteins: a potential tool for varietal differentiation. Journal of Agricultural and Food Chemistry, 49 4: 1830-1839. doi:10.1021/jf001163+


Author Hayasaka, Yoji
Adams, Kathryn S.
Pocock, Kenneth F.
Baldock, Gayle A.
Waters, Elizabeth J.
Hoj, Peter B.
Title Use of electrospray mass spectrometry for mass determination of grape (Vitis vinifera) juice pathogenesis-related proteins: a potential tool for varietal differentiation
Journal name Journal of Agricultural and Food Chemistry   Check publisher's open access policy
ISSN 0021-8561
1520-5118
Publication date 2001-04-01
Sub-type Article (original research)
DOI 10.1021/jf001163+
Volume 49
Issue 4
Start page 1830
End page 1839
Total pages 10
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Methods based on liquid chromatography - mass spectrometry (LC-MS) and protein trap mass spectrometry (trap-MS) were developed to determine the complement of pathogenesis-related (PR) proteins in grape juice. Trap-MS was superior to LC-MS in terms of simplicity, rapidity, and sensitivity. Proteins with a wide range of masses (13-33 kDa) were found in the juices of 19 different varieties of grape (Vitis vinifera) and were identified as mostly PR-5 type (thaumatin-like) and PR-3 type (chitinases) proteins. Although the PR proteins in juices of grapes are highly conserved, small consistent differences in molecular masses were noted when otherwise identical proteins were compared from different varieties. These differences persisted through different harvest years and in fruits grown in different Australian locations. With the definition of four different masses for PR-5 proteins (range = 21239-21272 Da) and nine different masses of PR-3 proteins (range = 25330-25631 Da) and using statistical analysis, the methods developed could be used for varietal differentiation of grapes grown in several South Australian locations on the basis of the PR protein composition of the juice. It remains to be seen whether this technology can be extended to grapes grown worldwide and to wine and other fruit-derived products to assist with label integrity to the benefit of consumers.
Keyword Grape proteins
Pathogenesis-related proteins
Thaumatin-like proteins
Chitinases
Varietal differentiation
LC-MS
Electrospray
Mass spectrometry
Vitis vinifera
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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