Proline accumulation in developing grapevine fruit occurs independently of changes in the levels of Delta(1)-pyrroline-5-carboxylate synthetase mRNA or protein

Stines, Anna P., Naylor, Dean J., Høj, Peter B. and van Heeswijck, Robyn (1999) Proline accumulation in developing grapevine fruit occurs independently of changes in the levels of Delta(1)-pyrroline-5-carboxylate synthetase mRNA or protein. Plant Physiology, 120 3: 923-931. doi:10.1104/pp.120.3.923


Author Stines, Anna P.
Naylor, Dean J.
Høj, Peter B.
van Heeswijck, Robyn
Title Proline accumulation in developing grapevine fruit occurs independently of changes in the levels of Delta(1)-pyrroline-5-carboxylate synthetase mRNA or protein
Formatted title
Proline accumulation in developing grapevine fruit occurs independently of changes in the levels of ∆1-pyrroline-5-carboxylate synthetase mRNA or protein
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
1532-2548
Publication date 1999-07
Sub-type Article (original research)
DOI 10.1104/pp.120.3.923
Volume 120
Issue 3
Start page 923
End page 931
Total pages 9
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Language eng
Abstract Mature fruit of grapevine (Vitis vinifera) contains unusually high levels of free proline (Pro; up to 24 μmol or 2.8 mg/g fresh weight). Pro accumulation does not occur uniformly throughout berry development but only during the last 4 to 6 weeks of ripening when both berry growth and net protein accumulation have ceased. In contrast, the steady-state levels of both the mRNA encoding V. viniferaΔ1-pyrroline-5-carboxylate synthetase (VVP5CS), a key regulatory enzyme in Pro biosynthesis, and its protein product remain relatively uniform throughout fruit development. In addition, the steady-state protein levels of Pro dehydrogenase, the first enzyme in Pro degradation, increased throughout early fruit development but thereafter remained relatively constant. The developmental accumulation of free Pro late in grape berry ripening is thus clearly distinct from the osmotic stress-induced accumulation of Pro in plants. It is not associated with either sustained increases in steady-state levels of P5CS mRNA or protein or a decrease in steady-state levels of Pro dehydrogenase protein, suggesting that other physiological factors are important for its regulation.
Keyword Arabidopsis thaliana
Osmotic stress
Water stress
2 steps
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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