Generation of a stable folding intermediate which can be rescued by the chaperonins GroEL and GroES

Peralta, Dadna, Hartman, Dallas J., Hoogenraad, Nicholas J. and Høj, Peter B. (1994) Generation of a stable folding intermediate which can be rescued by the chaperonins GroEL and GroES. FEBS Letters, 339 1-2: 45-49. doi:10.1016/0014-5793(94)80381-1


Author Peralta, Dadna
Hartman, Dallas J.
Hoogenraad, Nicholas J.
Høj, Peter B.
Title Generation of a stable folding intermediate which can be rescued by the chaperonins GroEL and GroES
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
1873-3468
Publication date 1994-02-14
Sub-type Article (original research)
DOI 10.1016/0014-5793(94)80381-1
Volume 339
Issue 1-2
Start page 45
End page 49
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Pig heart mitochondrial malate dehydrogenase was chemically denatured in guanidine HCl. Upon 50-fold dilution of the denaturant spontaneous refolding could be observed in the temperature range 12–32°C. At 36°C spontaneous refolding was not observed but a stable folding intermediate that is fairly resistant to aggregation was formed. This intermediate is readily refolded by the chaperonins GroEL and GroES and may prove useful in future attempts to describe several aspects of chaperonin action at physiological temperatures.
Keyword Malate dehydrogenase
Aggregation
Denaturation
Escherichia coli
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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