Identification of a GTP-binding protein in the contact sites between inner and outer mitochondrial membranes

Lithgow, Trevor, Timms, Mark, Høj, Peter B. and Hoogenraad, Nicholas J. (1991) Identification of a GTP-binding protein in the contact sites between inner and outer mitochondrial membranes. Biochemical and Biophysical Research Communications, 180 3: 1453-1459. doi:10.1016/S0006-291X(05)81359-2


Author Lithgow, Trevor
Timms, Mark
Høj, Peter B.
Hoogenraad, Nicholas J.
Title Identification of a GTP-binding protein in the contact sites between inner and outer mitochondrial membranes
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
1090-2104
Publication date 1991-11-14
Sub-type Article (original research)
DOI 10.1016/S0006-291X(05)81359-2
Volume 180
Issue 3
Start page 1453
End page 1459
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Whilst investigating whether GTP hydrolysis may be required for the import of preproteins into mitochondria we have found that a GTP-binding protein is located at the contact sites between mitochondrial inner and outer membranes. When mitochondrial outer membranes purified from rat liver were UV-irradiated in the presence of [α-32p]GTP, a 52 kDa protein was radiolabelled, whereas [α-32P]ATP did not label this protein. GTP-binding proteins were also labelled in the cytosolic and microsomal fractions, but the 52 kDa protein was concentrated in mitochondrial membranes and was the only protein specifically labelled by GTP in these membranes. Fractionation of mitochondrial membrane vesicles into outer membranes, inner membranes and contact sites between outer and inner membranes showed that the GTP-binding activity was highly enriched in contact sites, the location at which preprotein import is believed to occur. A protein of almost identical size was also found to be labelled in mitochondria from yeast.
Keyword Signal-recognition particle
Purified precursor protein
Import-receptor
Peptide
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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