Isolation of a cDNA clone specifying rat chaperonin-10, a stress-inducible mitochondrial matrix protein synthesized without a cleavable presequence

Ryan, Michael T., Hoogenraad, Nicholas J. and Høj, Peter B. (1994) Isolation of a cDNA clone specifying rat chaperonin-10, a stress-inducible mitochondrial matrix protein synthesized without a cleavable presequence. FEBS Letters, 337 2: 152-156. doi:10.1016/0014-5793(94)80263-7


Author Ryan, Michael T.
Hoogenraad, Nicholas J.
Høj, Peter B.
Title Isolation of a cDNA clone specifying rat chaperonin-10, a stress-inducible mitochondrial matrix protein synthesized without a cleavable presequence
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
1873-3468
Publication date 1994-01-10
Sub-type Article (original research)
DOI 10.1016/0014-5793(94)80263-7
Volume 337
Issue 2
Start page 152
End page 156
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract We have isolated a cDNA clone encoding chaperonin 10 from rat liver. The cDNA specifies a protein of 102 amino acids which, when transcribed and translated in vitro, yields a single basic product (pI > 9) that co-migrates exactly with the heat shock inducible cpn10 of rat hepatoma cells during 2D gel-electrophoresis. It is concluded that cpn10, unlike the majority of nuclear-encoded proteins of the mitochondrial matrix, is synthesised without a cleavable targeting signal and that, following removal of the initiating methionine, it becomes acetylated prior to mitochondrial import. Incubation of 3H- or 35S-labelled cpn10 with mitochondria confirms these conclusions and shows that cpn10 is imported into mitochondria in an energy-dependent process which is inhibited by the presence of 2,4-dinitrophenol.
Keyword Protein Import
Acetylation
Amphiphilic helix
Heat-shock protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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