Matrix metalloproteinases (MMPs) inhibitory effects of an octameric oligopeptide isolated from abalone Haliotis discus hannai

Nguyen, Van-Tinh, Qian, Zhong-Ji, Ryu, BoMi, Kim, Kil-Nam, Kim, Daekyung, Kim, Young-Mog, Jeon, You-Jin, Park, Won Sun, Choi, Il-Whan, Kim, Geun Hyung, Je, Jae-Young and Jung, Won-Kyo (2013) Matrix metalloproteinases (MMPs) inhibitory effects of an octameric oligopeptide isolated from abalone Haliotis discus hannai. Food Chemistry, 141 1: 503-509. doi:10.1016/j.foodchem.2013.03.038


Author Nguyen, Van-Tinh
Qian, Zhong-Ji
Ryu, BoMi
Kim, Kil-Nam
Kim, Daekyung
Kim, Young-Mog
Jeon, You-Jin
Park, Won Sun
Choi, Il-Whan
Kim, Geun Hyung
Je, Jae-Young
Jung, Won-Kyo
Title Matrix metalloproteinases (MMPs) inhibitory effects of an octameric oligopeptide isolated from abalone Haliotis discus hannai
Formatted title
Matrix metalloproteinases (MMPs) inhibitory effects of an octameric oligopeptide isolated from abalone Haliotis discus hannai
Journal name Food Chemistry   Check publisher's open access policy
ISSN 0308-8146
1873-7072
Publication date 2013-11-01
Sub-type Article (original research)
DOI 10.1016/j.foodchem.2013.03.038
Volume 141
Issue 1
Start page 503
End page 509
Total pages 7
Place of publication Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
Highlights
• Abalone, a marine gastropod has been investigated as bio-peptide sources.
• In this study, abalone oligopeptide (AOP) was purified from H. discus hannai.
• The AOP (782.4 Da) showed inhibition effect on MMP-2/-9 in HT1080 cell line.
• We demonstrated that AOP suppresses MMP-2/-9 via NF-κB pathway in HT1080 cell line.

Abalone (Haliotis discus hannai) is a marine gastropod, and an important fishery and food industrial resource that is massively maricultured in Asia, Africa, Australia and America. However, its health benefits have rarely been studied for nutraceutical and pharmaceutical application. In this study, the purified abalone oligopeptide (AOP) with anti-matrix metalloproteinases (anti-MMPs) effects was isolated from the digests of abalone intestine using recycle HPLC with a JAI W253 column and an OHpak SB-803 HQ column. The AOP was identified as Ala-Glu-Leu-Pro-Ser-Leu-Pro-Gly (MW = 782.4 Da) with a de novo peptide sequencing technique using a tandem mass spectrometer. The AOP exhibited a specific inhibitory effect against MMP-2/-9 activity and attenuated protein expression of p50 and p65 in the human fibrosarcoma (HT1080) cells, dose-dependently. The results presented illustrate that the AOP could inhibit MMP-2/-9 expression in HT1080 cells via the nuclear factor-kappaB (NF-κB)-mediated pathway. This data suggest that the AOP from H. discus hannai intestine may possess therapeutic and preventive potential for the treatment of MMPs-related disorders such as angiogenesis and cardiovascular diseases.
Keyword Matrix metalloproteinases (MMP-2/-9)
Human fibrosarcoma cells (HT1080)
Abalone Haliotis discus hannai
Purified abalone oligopeptide (AOP)
Nuclear factor-kappaB (NF-kappa B)
Spontaneously hypertensive-rats
Human fibrosarcoma cells
NF-kappa-B
Frame protein
Crassostrea-gigas
Cyclic Peptide
Purification
Hydrolysate
Antioxidant
Oyster
Nuclear factor-kappaB (NF-κB)
NF-κB
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Pharmacy Publications
 
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