Agrobacterium rhizogenes transformed soybean roots differ in their nodulation and nitrogen fixation response to genistein and salt stress

Dolatabadian, Aria, Sanavy, Seyed Ali Mohammad Modarres, Ghanati, Faezeh and Gresshoff, Peter M. (2013) Agrobacterium rhizogenes transformed soybean roots differ in their nodulation and nitrogen fixation response to genistein and salt stress. World Journal of Microbiology and Biotechnology, 29 7: 1327-1339. doi:10.1007/s11274-013-1296-2


Author Dolatabadian, Aria
Sanavy, Seyed Ali Mohammad Modarres
Ghanati, Faezeh
Gresshoff, Peter M.
Title Agrobacterium rhizogenes transformed soybean roots differ in their nodulation and nitrogen fixation response to genistein and salt stress
Formatted title
Agrobacterium rhizogenes transformed soybean roots differ in their nodulation and nitrogen fixation response to genistein and salt stress 
Journal name World Journal of Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0959-3993
1573-0972
Publication date 2013-07
Sub-type Article (original research)
DOI 10.1007/s11274-013-1296-2
Open Access Status
Volume 29
Issue 7
Start page 1327
End page 1339
Total pages 13
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2014
Language eng
Formatted abstract
We evaluated response differences of normal and transformed (so-called 'hairy') roots of soybean (Glycine max L. (Merr.), cv L17) to the Nod-factor inducing isoflavone genistein and salinity by quantifying growth, nodulation, nitrogen fixation and biochemical changes. Composite soybean plants were generated using Agrobacterium rhizogenes-mediated transformation of non-nodulating mutant nod139 (GmNFR5α minus) with complementing A. rhizogenes K599 carrying the wild-type GmNFR5α gene under control of the constitutive CaMV 35S promoter. We used genetic complementation for nodulation ability as only nodulated roots were scored. After hairy root emergence, primary roots were removed and composite plants were inoculated with Bradyrhizobium japonicum (strain CB1809) pre-induced with 10 μM genistein and watered with NaCl (0, 25, 50 and 100 mM). There were significant differences between hairy roots and natural roots in their responses to salt stress and genistein application. In addition, there were noticeable nodulation and nitrogen fixation differences. Composite plants had better growth, more root volume and chlorophyll as well as more nodules and higher nitrogenase activity (acetylene reduction) compared with natural roots. Decreased lipid peroxidation, proline accumulation and catalase/peroxidase activities were found in 'hairy' roots under salinity stress. Genistein significantly increased nodulation and nitrogen fixation and improved roots and shoot growth. Although genistein alleviated lipid peroxidation under salinity stress, it had no significant effect on the activity of antioxidant enzymes. In general, composite plants were more competitive in growth, nodulation and nitrogen fixation than normal non-transgenic even under salinity stress conditions.
Keyword Agrobacterium
Composite plants
Genistein
Hairy roots
Salt stress
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
Official 2014 Collection
 
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