Molecular characterization of endocarditis-associated Staphylococcus aureus

Nethercott, Cara, Mabbett, Amanda N., Totsika, Makrina, Peters, Paul, Ortiz, Juan C., Nimmo, Graeme R., Coombs, Geoffrey W., Walker, Mark J. and Schembri, Mark A. (2013) Molecular characterization of endocarditis-associated Staphylococcus aureus. Journal of Clinical Microbiology, 51 7: 2131-2138. doi:10.1128/JCM.00651-13

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Author Nethercott, Cara
Mabbett, Amanda N.
Totsika, Makrina
Peters, Paul
Ortiz, Juan C.
Nimmo, Graeme R.
Coombs, Geoffrey W.
Walker, Mark J.
Schembri, Mark A.
Title Molecular characterization of endocarditis-associated Staphylococcus aureus
Formatted title
 Molecular characterization of endocarditis-associated Staphylococcus aureus
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
Publication date 2013-07
Year available 2013
Sub-type Article (original research)
DOI 10.1128/JCM.00651-13
Open Access Status File (Publisher version)
Volume 51
Issue 7
Start page 2131
End page 2138
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2014
Language eng
Formatted abstract
Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gene microarrays are frequently used to classify S. aureus clinical isolates. This study examined the utility of these typing tools to investigate S. aureus epidemiology associated with IE. Ninetyseven S. aureus isolates were collected from patients diagnosed with (i) IE, (ii) bloodstream infection related to medical devices, (iii) bloodstream infection not related to medical devices, and (iv) skin or soft-tissue infections. The MLST clonal complex (CC) for each isolate was determined and compared to the CCs of members of the S. aureus population by eBURST analysis. The spa type of all isolates was also determined. A null model was used to determine correlations of IE with CC and spa type. DNA microarray analysis was performed, and a permutational analysis of multivariate variance (PERMANOVA) and principal coordinates analysis were conducted to identify genotypic differences between IE and non-IE strains. CC12, CC20, and spa type t160 were significantly associated with IE S. aureus. A subset of virulence-associated genes and alleles, including genes encoding staphylococcal superantigen-like proteins, fibrinogen-binding protein, and a leukocidin subunit, also significantly correlated with IE isolates. MLST, spa typing, and microarray analysis are promising tools for monitoring S. aureus epidemiology associated with IE. Further research to determine a role for the S. aureus IE-associated virulence genes identified in this study is warranted
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
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