Examination of the subsarcolemmal tubular system of mammalian skeletal muscle fibers

Jayasinghe, Isuru D., Lo, Harriet P., Morgan, Garry P., Baddeley, David, Parton, Robert G., Soeller, Christian and Launikonis, Bradley S. (2013) Examination of the subsarcolemmal tubular system of mammalian skeletal muscle fibers. Biophysical Journal, 104 11: L19-L21. doi:10.1016/j.bpj.2013.04.029


Author Jayasinghe, Isuru D.
Lo, Harriet P.
Morgan, Garry P.
Baddeley, David
Parton, Robert G.
Soeller, Christian
Launikonis, Bradley S.
Title Examination of the subsarcolemmal tubular system of mammalian skeletal muscle fibers
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
1542-0086
Publication date 2013-06
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.bpj.2013.04.029
Volume 104
Issue 11
Start page L19
End page L21
Total pages 3
Place of publication St. Louis, MO, United States
Publisher Cell Press
Collection year 2014
Language eng
Abstract A subsarcolemmal tubular system network (SSTN) has been detected in skeletal muscle fibers by confocal imaging after the removal of the sarcolemma. Here we confirm the existence and resolve the fine architecture and the localization of the SSTN at an unprecedented level of detail by examining extracellularly applied tubular system markers in skeletal muscle fiber preparations with a combination of three imaging modalities: confocal fluorescence microscopy, direct stochastic optical reconstruction microscopy, and tomographic electron microscopy. Three-dimensional reconstructions showed that the SSTN was a dense two-dimensional network within the subsarcolemmal space around the fiber, running ∼500-600 nm underneath and parallel to the sarcolemma. The SSTN is composed of tubules ∼95 nm in width with ∼60% of the tubules directed transversely and >30% directed longitudinally. The deeper regular transverse tubules located at each A-I boundary of the sarcomeres branched from the SSTN, indicating individual transverse tubules that form triads are continuous with, but do not directly contact the sarcolemma. This suggests that the SSTN plays an important role in affecting the exchange of deeper tubule lumina with the extracellular space.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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