Previously unclassified bacteria dominate during thermophilic and mesophilic anaerobic pre-treatment of primary sludge

Pervin, Hasina M., Batstone, Damien J. and Bond, Philip L. (2013) Previously unclassified bacteria dominate during thermophilic and mesophilic anaerobic pre-treatment of primary sludge. Systematic and Applied Microbiology, 36 4: 281-290. doi:10.1016/j.syapm.2013.03.003


Author Pervin, Hasina M.
Batstone, Damien J.
Bond, Philip L.
Title Previously unclassified bacteria dominate during thermophilic and mesophilic anaerobic pre-treatment of primary sludge
Journal name Systematic and Applied Microbiology   Check publisher's open access policy
ISSN 0723-2020
1618-0984
Publication date 2013-06
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.syapm.2013.03.003
Volume 36
Issue 4
Start page 281
End page 290
Total pages 10
Place of publication Jena, Germany
Publisher Urban und Fischer Verlag
Collection year 2014
Language eng
Formatted abstract
Thermophilic biological pre-treatment enables enhanced anaerobic digestion for treatment of wastewater sludges but, at present, there is limited understanding of the hydrolytic-acidogenic microbial composition and its contribution to this process. In this study, the process was assessed by comparing the microbiology of thermophilic (50-65. °C) and mesophilic (35. °C) pre-treatment reactors treating primary sludge.A full-cycle approach for the 16S rRNA genes was applied in order to monitor the diversity of bacteria and their abundance in a thermophilic pre-treatment reactor treating primary sludge. For the thermophilic pre-treatment (TP), over 90% of the sequences were previously undetected and these had less than 97% sequence similarity to cultured organisms. During the first 83 days, members of the Betaproteobacteria dominated the community sequences and a newly designed probe was used to monitor a previously unknown bacterium affiliated with the genus Brachymonas. Between days 85 and 183, three phylotypes that affiliated with the genera Comamonas, Clostridium and Lysobacter were persistently dominant in the TP community, as revealed by terminal-restriction fragment length polymorphism (T-RFLP). Hydrolytic and fermentative functions have been speculated for these bacteria. Mesophilic pre-treatment (MP) and TP communities were different but they were both relatively dynamic.Statistical correlation analysis and the function of closely allied reference organisms indicated that previously unclassified bacteria dominated the TP community and may have been functionally involved in the enhanced hydrolytic performance of thermophilic anaerobic pre-treatment. This study is the first to reveal the diversity and dynamics of bacteria during anaerobic digestion of primary sludge.
Keyword Anaerobic digestion
Microbial diversity
Fluorescence in situ hybridisation (FISH)
Terminal restriction fragment length polymorphism (T RFLP)
Thermophilic pre-treatment
Hydrolysis fermentation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Advanced Water Management Centre Publications
 
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