Global transcriptome profiles of Camellia sinensis during cold acclimation

Wang, Xin-Chao, Zhao, Qiong-Yi, Ma, Chun-Lei, Zhang, Zong-Hong, Cao, Hong-Li, Kong, Yi-Meng, Yue, Chuan, Hao, Xin-Yuan, Chen, Liang, Ma, Jian-Qiang, Jin, Ji-Qiang, Li, Xuan and Yang, Ya-Jun (2013) Global transcriptome profiles of Camellia sinensis during cold acclimation. BMC Genomics, 14 . doi:10.1186/1471-2164-14-415

Author Wang, Xin-Chao
Zhao, Qiong-Yi
Ma, Chun-Lei
Zhang, Zong-Hong
Cao, Hong-Li
Kong, Yi-Meng
Yue, Chuan
Hao, Xin-Yuan
Chen, Liang
Ma, Jian-Qiang
Jin, Ji-Qiang
Li, Xuan
Yang, Ya-Jun
Title Global transcriptome profiles of Camellia sinensis during cold acclimation
Formatted title
Global transcriptome profiles of Camellia sinensis during cold acclimation
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2013-06
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1471-2164-14-415
Open Access Status DOI
Volume 14
Total pages 14
Place of publication London, United Kingdom
Publisher BioMed
Collection year 2014
Language eng
Formatted abstract
Tea is the most popular non-alcoholic health beverage in the world. The tea plant (Camellia sinensis (L.) O. Kuntze) needs to undergo a cold acclimation process to enhance its freezing tolerance in winter. Changes that occur at the molecular level in response to low temperatures are poorly understood in tea plants. To elucidate the molecular mechanisms of cold acclimation, we employed RNA-Seq and digital gene expression (DGE) technologies to the study of genome-wide expression profiles during cold acclimation in tea plants.

Using the Illumina sequencing platform, we obtained approximately 57.35 million RNA-Seq reads. These reads were assembled into 216,831 transcripts, with an average length of 356 bp and an N50 of 529 bp. In total, 1,770 differentially expressed transcripts were identified, of which 1,168 were up-regulated and 602 down-regulated. These include a group of cold sensor or signal transduction genes, cold-responsive transcription factor genes, plasma membrane stabilization related genes, osmosensing-responsive genes, and detoxification enzyme genes. DGE and quantitative RT-PCR analysis further confirmed the results from RNA-Seq analysis. Pathway analysis indicated that the “carbohydrate metabolism pathway” and the “calcium signaling pathway” might play a vital role in tea plants’ responses to cold stress.

Our study presents a global survey of transcriptome profiles of tea plants in response to low, non-freezing temperatures and yields insights into the molecular mechanisms of tea plants during the cold acclimation process. It could also serve as a valuable resource for relevant research on cold-tolerance and help to explore the cold-related genes in improving the understanding of low-temperature tolerance and plant-environment interactions.
Keyword Camellia sinensis
Cold acclimation
Genome-wide expression profiles
Tea plants
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 415

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 53 times in Thomson Reuters Web of Science Article | Citations
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