Na+ extrusion imposes an acid load on the intraerythrocytic malaria parasite

Spillman, Natalie J., Allen, Richard J. W. and Kirk, Kiaran (2013) Na+ extrusion imposes an acid load on the intraerythrocytic malaria parasite. Molecular and Biochemical Parasitology, 189 1-2: 1-4. doi:10.1016/j.molbiopara.2013.04.004


Author Spillman, Natalie J.
Allen, Richard J. W.
Kirk, Kiaran
Title Na+ extrusion imposes an acid load on the intraerythrocytic malaria parasite
Formatted title
Na+ extrusion imposes an acid load on the intraerythrocytic malaria parasite
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
1872-9428
Publication date 2013-05
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.molbiopara.2013.04.004
Open Access Status
Volume 189
Issue 1-2
Start page 1
End page 4
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
The intraerythrocytic malaria parasite has, on its plasma membrane, a H+-extruding V-type H+-ATPase that plays a central role in maintaining the resting cytosolic pH at around 7.3. Previous studies have demonstrated the presence in the parasite of an unknown acidification mechanism that is revealed on inhibition of the V-type H+-ATPase. Here we show that this acidification is dependent on the presence of extracellular Na+, and is associated with the activity of a plasma membrane Na+-ATPase that is inhibited by the novel antimalarial spiroindolone NITD246 and is postulated to export Na+ ions in counter-transport with H+ ions. The proposed import of H+ by the Na+-extruding Na+-ATPase necessitates “abundant H+ pumping” by the V-type H+-ATPase (Ginsburg H. Abundant proton pumping in Plasmodium falciparum, but why? Trends in Parasitology 2002;18:483–6) and has significant implications for the energy budget of the parasite.
Keyword Na+ homeostasis
ATPase
PfATP4
ENA
Pump
Transporter
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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