Diversity of sequences and expression patterns among alleles of a sugarcane loading stem gene

Moyle, Richard L. and Birch, Robert G. (2013) Diversity of sequences and expression patterns among alleles of a sugarcane loading stem gene. Theoretical and Applied Genetics, 126 7: 1775-1782. doi:10.1007/s00122-013-2091-z


Author Moyle, Richard L.
Birch, Robert G.
Title Diversity of sequences and expression patterns among alleles of a sugarcane loading stem gene
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
1432-2242
Publication date 2013-07
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s00122-013-2091-z
Volume 126
Issue 7
Start page 1775
End page 1782
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2014
Language eng
Formatted abstract
Modern sugarcane cultivars are highly polyploid and aneuploid hybrids, which are propagated as clones. Their complex genome structure comprises 100–130 chromosomes and 10–13 hom(e)ologous copies of most loci. There is preliminary evidence of very high heterozygosity, with implications for genetic improvement approaches ranging from marker-assisted selection to transgenics. Here, we report that sugarcane cultivar Q200 has at least nine alleles at the Loading Stem Gene (ScLSG) locus. Exon–intron structure is identical and the predicted protein products show at least 92 % identity, across sugarcane alleles and the Sorghum homologue Sb07g027880. There is substantial variation in the 5′ UTR and promoter regions including numerous allele-specific nucleotide polymorphisms, insertions and deletions. We developed an allele-specific qRT-PCR method to undertake the first compelling test of allele-specific expression in polyploid sugarcane. Seven alleles distinguished by this method all showed peak expression in the sucrose-loading zone of the stem, but there was apparent variability in expression patterns across other tissues. The ScLSG2 and ScLSG5 alleles appear promising for specificity of expression in stems, relative to leaf, meristem, emerging shoot and root tissues. Within the stem, there was activity in parenchyma, vascular and rind tissues. This expression pattern is of interest in basic research and biotechnology aimed at enhanced sucrose content, engineering value-added products, and manipulation of stem biomass composition.
Keyword Genome evolution
Polyploid plant
Brassica napus
Polymorphism
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 11 Aug 2013, 00:03:29 EST by System User on behalf of Scholarly Communication and Digitisation Service