Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release

Christensen, Melinda E., Jansen, Elisa S., Sanchez, Washington and Waterhouse, Nigel J. (2013) Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release. Methods, 61 2: 138-145. doi:10.1016/j.ymeth.2013.03.020


Author Christensen, Melinda E.
Jansen, Elisa S.
Sanchez, Washington
Waterhouse, Nigel J.
Title Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release
Formatted title
Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release
Journal name Methods   Check publisher's open access policy
ISSN 1046-2023
1095-9130
Publication date 2013-06
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.ymeth.2013.03.020
Open Access Status
Volume 61
Issue 2
Start page 138
End page 145
Total pages 8
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2014
Language eng
Formatted abstract
Mitochondria play a pivotal role in life and death of the cell because they produce the majority of energy required for survival and also regulate the intrinsic pathway to apoptosis. The involvement of mitochondria in cell death is generally measured by following mitochondrial membrane depolarisation or mitochondrial outer membrane permeabilisation (MOMP). These events can be assayed using cationic dyes that are attracted to the negative charge across the inner membrane of healthy mitochondria or by following translocation of cytochrome c from the mitochondria to the cytoplasm respectively. These events progress rapidly in individual cells but are observed as bi-phasic peaks in flow cytometry assays because cell death generally occurs asynchronously in a population. This allows researchers to use flow cytometry to easily distinguish healthy cells with intact mitochondria healthy from dying cells with permeabilised mitochondria. This article will therefore review methods using flow cytometry to follow mitochondrial membrane depolarisation and cytochrome c release during apoptosis, and will highlight some studies that resulted in development of these assays.
Keyword Mitochondria
Apoptosis
Cytochrome c
Membrane potential
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
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