MicroRNAs-140-5p/140-3p modulate leydig cell numbers in the developing mouse testis

Rakoczy, Joanna, Fernandez-Valverde, Selene L., Glazov, Evgeny A., Wainwright, Elanor N., Sato, Tempei, Takada, Shuji, Combes, Alexander N., Korbie, Darren J., Miller, David, Grimmond, Sean M., Little, Melissa H., Asahara, Hiroshi, Mattick, John S., Taft, Ryan J. and Wilhelm, Dagmar (2013) MicroRNAs-140-5p/140-3p modulate leydig cell numbers in the developing mouse testis. Biology of Reproduction, 88 6: . doi:10.1095/biolreprod.113.107607

Author Rakoczy, Joanna
Fernandez-Valverde, Selene L.
Glazov, Evgeny A.
Wainwright, Elanor N.
Sato, Tempei
Takada, Shuji
Combes, Alexander N.
Korbie, Darren J.
Miller, David
Grimmond, Sean M.
Little, Melissa H.
Asahara, Hiroshi
Mattick, John S.
Taft, Ryan J.
Wilhelm, Dagmar
Title MicroRNAs-140-5p/140-3p modulate leydig cell numbers in the developing mouse testis
Formatted title
MicroRNAs-140-5p/140-3p modulate leydig cell numbers in the developing mouse testis
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 2013-06-01
Year available 2013
Sub-type Article (original research)
DOI 10.1095/biolreprod.113.107607
Volume 88
Issue 6
Total pages 11
Place of publication Madison, WI, United States
Publisher Society for the Study of Reproduction
Collection year 2014
Language eng
Formatted abstract
MicroRNAs (miRNAs) have been shown to play key regulatory roles in a range of biological processes, including cell differentiation and development. To identify miRNAs that participate in gonad differentiation, a fundamental and tightly regulated developmental process, we examined miRNA expression profiles at the time of sex determination and during the early fetal differentiation of mouse testes and ovaries using high-throughput sequencing. We identified several miRNAs that were expressed in a sexually dimorphic pattern, including several members of the let-7 family, miR-378, and miR-140-3p. We focused our analysis on the most highly expressed, sexually dimorphic miRNA, miR-140-3p, and found that both miR-140-3p and its more lowly expressed counterpart, the previously annotated guide strand, miR-140-5p, are testis enriched and expressed in testis cords. Analysis of the miR-140-5p/miR-140-3p-null mouse revealed a significant increase in the number of Leydig cells in the developing XY gonad, strongly suggesting an important role for miR-140-5p/miR-140-3p in testis differentiation in mouse.
Keyword Gonad development
High-throughput sequencing
Sex determination
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 143

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
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