Using a Virus-Derived System to Manipulate Plant Natural Product Biosynthetic Pathways

Sainsbury, Frank, Saxena, Pooja, Geisler, Katrin, Osbourn, Anne and Lomonossoff, George P. (2012) Using a Virus-Derived System to Manipulate Plant Natural Product Biosynthetic Pathways. Methods in Enzymology, 517 185-202. doi:10.1016/B978-0-12-404634-4.00009-7


Author Sainsbury, Frank
Saxena, Pooja
Geisler, Katrin
Osbourn, Anne
Lomonossoff, George P.
Title Using a Virus-Derived System to Manipulate Plant Natural Product Biosynthetic Pathways
Journal name Methods in Enzymology   Check publisher's open access policy
ISSN 0076-6879
1557-7988
ISBN 9780124046344
Publication date 2012-01
Year available 2012
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/B978-0-12-404634-4.00009-7
Open Access Status
Volume 517
Start page 185
End page 202
Total pages 18
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2012
Language eng
Formatted abstract
 A series of vectors (the pEAQ series) based on cowpea mosaic virus has been developed which allows the rapid transient expression of high levels of foreign protein in plants without the need for viral replication. The plasmids are small binary vectors, which are introduced into plant leaves by agroinfiltration. They are modular in design and allow the insertion of multiple coding sequences on the same segment of T-DNA. These properties make the pEAQ vectors particularly suitable for use in situations, such as the investigation and manipulation of metabolic pathways, where the coexpression of multiple proteins within a cell is required.
Keyword Cowpea mosaic virus
Transient Expression
Foreign Proteins
Vectors
Identification
Antibodies
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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