Beneficial 'unintended effects' of a cereal cystatin in transgenic lines of potato, Solanum tuberosum

Munger, Aurelie, Coenen, Karine, Cantin, Line, Goulet, Charles, Vaillancourt, Louis-Philippe, Goulet, Marie-Claire, Tweddell, Russell, Sainsbury, Frank and Michaud, Dominique (2012) Beneficial 'unintended effects' of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biology, 12 . doi:10.1186/1471-2229-12-198

Author Munger, Aurelie
Coenen, Karine
Cantin, Line
Goulet, Charles
Vaillancourt, Louis-Philippe
Goulet, Marie-Claire
Tweddell, Russell
Sainsbury, Frank
Michaud, Dominique
Title Beneficial 'unintended effects' of a cereal cystatin in transgenic lines of potato, Solanum tuberosum
Journal name BMC Plant Biology   Check publisher's open access policy
ISSN 1471-2229
Publication date 2012-11
Year available 2012
Sub-type Article (original research)
DOI 10.1186/1471-2229-12-198
Open Access Status DOI
Volume 12
Total pages 12
Place of publication London, United Kingdom
Publisher BioMed Central Ltd.
Collection year 2012
Language eng
Formatted abstract
Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects in planta remain poorly understood. Here we assessed the effects of corn cystatin II (CCII), a potent inhibitor of C1A cysteine (Cys) proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein.

The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental) line. Out of ca. 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leaves, including 14 proteins detected de novo or up-regulated by more than five-fold compared to the control. Most up-regulated proteins were abiotic or biotic stress-responsive proteins, including different secretory peroxidases, wound inducible protease inhibitors and pathogenesis-related proteins. Accordingly, infection of leaf tissues by the fungal necrotroph Botryris cinerea was prevented in CCII-expressing plants, despite a null impact of CCII on growth of this pathogen and the absence of extracellular Cys protease targets for the inhibitor.

These data point to the onset of pleiotropic effects altering the leaf proteome in transgenic plants expressing recombinant protease inhibitors. They also show the potential of these proteins as ectopic modulators of stress responses in planta, useful to engineer biotic or abiotic stress tolerance in crop plants of economic significance. 
Keyword Transgenic crops
Transgene pleiotropy
Unintended effects
Stress/defense related proteome
Corn cystatin
Potato (Solanum tuberosum)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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