Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms

Ali, Syed A., Baumann, Kate, Jackson, Timothy N. W., Wood, Kelly, Mason, Steven, Undheim, Eivind A.B., Nouwens, Amanda, Koludarov, Ivan, Hendrikx, Iwan, Jones, Alun and Fry, Bryan Grieg (2013) Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms. Journal of Proteomics, 91 338-343. doi:10.1016/j.jprot.2013.07.020


Author Ali, Syed A.
Baumann, Kate
Jackson, Timothy N. W.
Wood, Kelly
Mason, Steven
Undheim, Eivind A.B.
Nouwens, Amanda
Koludarov, Ivan
Hendrikx, Iwan
Jones, Alun
Fry, Bryan Grieg
Title Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms
Formatted title
Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2013-10-08
Sub-type Article (original research)
DOI 10.1016/j.jprot.2013.07.020
Volume 91
Start page 338
End page 343
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
Treatment of Hypnale hypnale bites with commercial antivenoms, even those raised against its sister taxon Calloselasma rhodostoma, has never been clinically successful. As these two genera have been separated for 20 million years, we tested to see whether significant variations in venom had accumulated during this long period of evolutionary divergence, and thus could be responsible for the failure of antivenom. Proteomic analyses of C. rhodostoma and H. hypnale venom were performed using 1D and 2D PAGE as well as 2D-DIGE. C. rhodostoma venom was diverse containing large amounts of Disintegrin, Kallikrein, L-amino acid oxidase, Lectin, phospholipase A2 (acidic, basic and neutral) and Snake Venom Metalloprotease. In contrast, while H. hypnale also contained a wide range of toxin types, the venom was overwhelmingly dominated by two molecular weight forms of basic PLA2. 2D-DIGE (2-D Fluorescence Difference Gel Electrophoresis analysis) showed that even when a particular toxin class was shared between the two venoms, there were significant molecular weights or isoelectric point differences. This proteomic difference explains the past treatment failures with C. rhodostoma antivenom and highlights the need for a H. hypnale specific antivenom.

Biological significance
These results have direct implications for the treatment of envenomed patients in Sri Lanka. The unusual venom profile of Hypnale hypnale underscores the biodiscovery potential of novel snake venoms.
Keyword Venom
2D gel DiGE
Antivenom
Proteomics
Mass spectrometry
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 09 Aug 2013, 11:24:46 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences