The effect of experimentally induced chronic hyperglycaemia on serum and pancreatic insulin, pancreatic islet IGF-I and plasma and urinary ketones in the domestic cat (Felis felis)

Link, Karl R. J., Allio, Ileana, Rand, Jacqueline S. and Eppler, Elisabeth (2013) The effect of experimentally induced chronic hyperglycaemia on serum and pancreatic insulin, pancreatic islet IGF-I and plasma and urinary ketones in the domestic cat (Felis felis). General and Comparative Endocrinology, 188 1: 269-281. doi:10.1016/j.ygcen.2013.04.029


Author Link, Karl R. J.
Allio, Ileana
Rand, Jacqueline S.
Eppler, Elisabeth
Title The effect of experimentally induced chronic hyperglycaemia on serum and pancreatic insulin, pancreatic islet IGF-I and plasma and urinary ketones in the domestic cat (Felis felis)
Formatted title
The effect of experimentally induced chronic hyperglycaemia on serum and pancreatic insulin, pancreatic islet IGF-I and plasma and urinary ketones in the domestic cat (Felis felis)
Journal name General and Comparative Endocrinology   Check publisher's open access policy
ISSN 0016-6480
1095-6840
Publication date 2013-07-01
Sub-type Article (original research)
DOI 10.1016/j.ygcen.2013.04.029
Volume 188
Issue 1
Start page 269
End page 281
Total pages 13
Place of publication United States
Publisher Academic Press
Collection year 2014
Language eng
Formatted abstract
Highlights
• Diabetes mellitus is on the rise in domestic cats and resembles T2D in humans.
• We experimentally induced insulin suppression in serum and β-cells of healthy cats.
• We observed plasma and urinary ketones related to the induced insulin suppression.
• We for the first time localize IGF-I in distinct feline islet cells of Langerhans.
• We propose a protective effect of IGF-I within the β-cells against hyperglycaemia.

Like in humans, diabetes mellitus is on the rise in cats. Feline diabetes is a suitable model for human type-2 diabetes. We investigated magnitude and timing of insulin suppression with induced hyperglycaemia and its relationship to plasma and urinary ketones and to pancreatic islet insulin. IGF-I is under discussion as a protective mechanism but little is known about its role in diabetes in general and its distinct localisation in feline pancreatic islets in particular. Thirteen healthy, adult cats were allocated to 2 groups and infused with glucose to maintain their blood glucose at a high or moderate concentration for 42 days resulting in insulin secretion suppression. After initial increase, insulin levels declined to baseline but were still detectable in the blood at a very low level after 6 weeks of glucose infusion and then increased after a 3 week recovery period. While IGF-I in healthy cats was primarily located in glucagon cells, in hyperglycaemia-challenge IGF-I was pronounced in the β-cells 3 weeks after ceasation of infusion. Six/8 cats developing glucose toxicity became ketonuric after 3–4 weeks. Gross lipaemia occurred approx 1 week prior to ketonuria. Ketonuric cats required 1–2 weeks of insulin therapy after-infusion until β-cell recovery. In conclusion, ketosis and hyperlipidaemia are likely to occur in diabetic cats with glucose at 30 mmol/L, especially after ⩾2 weeks. Three weeks after ceasation of infusions, clinical and morphological recovery occurred. We propose a local protective effect of IGF-I to support survival and insulin production in the hyperglycaemic state and recovery period.
Keyword Glucose toxicity
Diabetes
Feline
Insulin
Glucagon
IGF-I
Growth-factor-I
Diabetes-mellitus
Endocrine pancreas
Glucose-tolerance
Bony fish
Immunohistochemical localization
Expression
Hormone
Tissue
Cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 28 Jul 2013, 00:03:25 EST by System User on behalf of School of Veterinary Science