Glioma surgical aspirate: a viable source of tumor tissue for experimental research

Day, Bryan W., Stringer, Brett W., Wilson, John, Jeffree, Rosalind L., Jamieson, Paul R., Ensbey, Kathleen S., Bruce, Zara C., Inglis, Po, Allan, Suzanne, Winter, Craig, Tollesson, Gert, Campbell, Scott, Lucas, Peter, Findlay, Wendy, Kadrian, David, Johnson, David, Robertson, Thomas, Johns, Terrance G., Bartlett, Perry F., Osborne, Geoffrey W. and Boyd, Andrew W. (2013) Glioma surgical aspirate: a viable source of tumor tissue for experimental research. Cancers, 5 2: 357-371. doi:10.3390/cancers5020357

Author Day, Bryan W.
Stringer, Brett W.
Wilson, John
Jeffree, Rosalind L.
Jamieson, Paul R.
Ensbey, Kathleen S.
Bruce, Zara C.
Inglis, Po
Allan, Suzanne
Winter, Craig
Tollesson, Gert
Campbell, Scott
Lucas, Peter
Findlay, Wendy
Kadrian, David
Johnson, David
Robertson, Thomas
Johns, Terrance G.
Bartlett, Perry F.
Osborne, Geoffrey W.
Boyd, Andrew W.
Title Glioma surgical aspirate: a viable source of tumor tissue for experimental research
Journal name Cancers   Check publisher's open access policy
ISSN 2072-6694
Publication date 2013-04-03
Year available 2013
Sub-type Article (original research)
DOI 10.3390/cancers5020357
Open Access Status DOI
Volume 5
Issue 2
Start page 357
End page 371
Total pages 15
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2014
Language eng
Formatted abstract
Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures.
Keyword Glioblastoma
Brain cancer
Q-Index Code C1
Q-Index Status Confirmed Code
Funding Body N/A
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2014 Collection
School of Medicine Publications
Version Filter Type
Citation counts: Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Tue, 23 Jul 2013, 15:04:33 EST by Debra McMurtrie on behalf of Queensland Brain Institute