Synergistic and antagonistic effects of alpha-amylase and amyloglucosidase on starch digestion

Zhang, Bin, Dhital, Sushi and Gidley, Michael J. (2013) Synergistic and antagonistic effects of alpha-amylase and amyloglucosidase on starch digestion. Biomacromolecules, 14 6: 1945-1954. doi:10.1021/bm400332a


Author Zhang, Bin
Dhital, Sushi
Gidley, Michael J.
Title Synergistic and antagonistic effects of alpha-amylase and amyloglucosidase on starch digestion
Formatted title
Synergistic and antagonistic effects of α-amylase and amyloglucosidase on starch digestion
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
1526-4602
Publication date 2013-06-10
Sub-type Article (original research)
DOI 10.1021/bm400332a
Volume 14
Issue 6
Start page 1945
End page 1954
Total pages 10
Place of publication United States
Publisher American Chemical Society
Collection year 2014
Language eng
Formatted abstract
The influence of biomacromolecular physical structure on the kinetics of degradation with exo-acting or a mixture of endo- and exo-acting enzymes was studied using three physical forms of maize and potato starch, amyloglucosidase (exo-acting) and α-amylase (endo-acting) as exemplars. For starch in granular form, there was synergism between the enzymes in the production of glucose. In contrast, endo- and exo-acting enzymes showed antagonistic effects in digestion of cooked starches. Antagonism was ascribed to the rapid production of low molecular weight oligomers by α-amylase, which are less efficiently digested by amyloglucosidase than polymeric substrates. The rates of digestion of swollen granule ghosts cooked under low shear conditions were slower than starches cooked under high shear conditions that prevent granule ghost formation. There was also an enzyme-resistant fraction present in granule ghosts, in contrast to high shear cooked starches that were fully digested under the conditions used.
Keyword Aspergillus-niger glucoamylase
Native cereal starches
Glycemic index
Enzymatic-hydrolysis
Granule structure
Maize starches
Amylose
Kinetics
Mechanism
Digestibility
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 29 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 14 Jul 2013, 00:07:00 EST by System User on behalf of Centre for Nutrition and Food Sciences