Pharmacological dissection of G protein-mediated second messenger cascades in neurons

Bellingham, Mark C. (2013). Pharmacological dissection of G protein-mediated second messenger cascades in neurons. In Pilowsky, Paul M., Farnham, Melissa M. J. and Fong, Angelina Y. (Ed.), Stimulation and inhibition of neurons (pp. 61-106) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-233-9_5


Author Bellingham, Mark C.
Title of chapter Pharmacological dissection of G protein-mediated second messenger cascades in neurons
Title of book Stimulation and inhibition of neurons
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Chapter in textbook
DOI 10.1007/978-1-62703-233-9_5
Year available 2013
Series Neuromethods
ISBN 9781627032322
9781627032339
ISSN 0893-2336
1940-6045
Editor Pilowsky, Paul M.
Farnham, Melissa M. J.
Fong, Angelina Y.
Chapter number 5
Start page 61
End page 106
Total pages 46
Total chapters 18
Collection year 2014
Language eng
Abstract/Summary Despite the advent of transgenic gene manipulation in vivo and in vitro and the rapid rise of RNA interference, in order to target protein function by ablation or suppression of specific gene products, pharmacological manipulation of G protein-coupled signalling pathways remains a highly useful tool for understanding receptor coupling to effector mechanisms in neurons. The key to drawing valid conclusions from pharmacological manipulations is a solid knowledge of the signalling pathway molecules and on- and off-target effects of the pharmacological tools to be used. This chapter outlines the actions, application and pitfalls of pharmacological ligands commonly used to dissect the major second messenger signalling cascades activated by G proteins in neurons. Despite the limitations of many currently used pharmacological tools, careful and judicious use of these tools allows the exploration and definition of these pathways at all levels.
Keyword G protein
Second messenger
Cyclic AMP
Cyclic GMP
Phospholipase C
Phospholipase A2
Arachidonic acid
Protein kinase
Protein phosphatase
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Book Chapter
Collections: Non HERDC
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Created: Thu, 27 Jun 2013, 18:17:05 EST by Dr Mark Bellingham on behalf of School of Biomedical Sciences