Cutinase-like protein-6 of Mycobacterium tuberculosis is recognised in tuberculosis patients and protects mice against pulmonary infection as a single and fusion protein vaccine

Shanahan, Erin R., Pinto, Rachel, Triccas, James A., Britton, Warwick J. and West, Nicholas P. (2010) Cutinase-like protein-6 of Mycobacterium tuberculosis is recognised in tuberculosis patients and protects mice against pulmonary infection as a single and fusion protein vaccine. Vaccine, 28 5: 1341-1346. doi:10.1016/j.vaccine.2009.11.010


Author Shanahan, Erin R.
Pinto, Rachel
Triccas, James A.
Britton, Warwick J.
West, Nicholas P.
Title Cutinase-like protein-6 of Mycobacterium tuberculosis is recognised in tuberculosis patients and protects mice against pulmonary infection as a single and fusion protein vaccine
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
Publication date 2010-02
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2009.11.010
Open Access Status
Volume 28
Issue 5
Start page 1341
End page 1346
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier Ltd
Collection year 2010
Language eng
Formatted abstract
 Infection with Mycobacterium tuberculosis continues to be a leading cause of death in many regions of the world, and control of this disease is hampered by the lack of a safe and effective vaccine. Secreted proteins of M. tuberculosis are an important group of antigens for subunit vaccines which target this infection. We have tested three secreted members of the cutinase-like protein (CULP) family of M. tuberculosis for their potential as protein vaccine antigens. Culp6 elicited a strong T lymphocyte response in M. tuberculosis infected mice, and importantly, in tuberculosis (TB) patients tested. Culp1, Culp2 and Culp6 when delivered as protein vaccines to mice, induced potent IFN-γ responses which in turn translated into a significant level of protection against aerosol M. tuberculosis infection. A Culp1–6 fusion protein provided an increased level of protection against infection compared to Culp1 or Culp6 alone. The data presented here may indicate that the cell wall-associated, putatively essential protein Culp6, shown here for the first time to be recognised in TB patients, is an attractive candidate for inclusion in future subunit vaccines.
Keyword Fusion protein vaccine
Tuberculosis (TB) patients
Boosting vaccine
Bacille Calmette Guerin
Culture Filtrate
Subunit Vaccine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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