Invasion pathways and malaria severity in Kenyan Plasmodium falciparum clinical isolates

Deans, Anne-Marie, Nery, Susana, Conway, David J., Kai, Oscar, Marsh, Kevin and Rowe, J. Alexandra (2007) Invasion pathways and malaria severity in Kenyan Plasmodium falciparum clinical isolates. Infection and Immunity, 75 6: 3014-3020. doi:10.1128/IAI.00249-07

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Author Deans, Anne-Marie
Nery, Susana
Conway, David J.
Kai, Oscar
Marsh, Kevin
Rowe, J. Alexandra
Title Invasion pathways and malaria severity in Kenyan Plasmodium falciparum clinical isolates
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2007-06
Sub-type Article (original research)
DOI 10.1128/IAI.00249-07
Open Access Status File (Publisher version)
Volume 75
Issue 6
Start page 3014
End page 3020
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The invasion of erythrocytes byPlasmodium falciparum occurs through multiple pathways that can be studied in vitro by examining the invasion of erythrocytes treated with enzymes such as neuraminidase, trypsin, and chymotrypsin. We have studied the invasion pathways used by 31 Kenyan P. falciparum isolates from children with uncomplicated or severe malaria. Six distinct invasion profiles were detected, out of eight possible profiles. The majority of isolates (23 of 31) showed neuraminidase-resistant, trypsin-sensitive invasion, characteristic of the pathway mediated by an unknown parasite ligand and erythrocyte receptor "X." The neuraminidase-sensitive, trypsin-sensitive phenotype consistent with invasion mediated by the binding of parasite ligand erythrocyte binding antigen 175 to glycophorin A, the most common invasion profile in a previous study of Gambian field isolates, was seen in only 3 of 31 Kenyan isolates. No particular invasion profile was associated with severe P. falciparum malaria, and there was no significant difference in the levels of inhibition by the various enzyme treatments between isolates from children with severe malaria and those from children with uncomplicated malaria (P, >0.1 for all enzymes; Mann-Whitney U test). These results do not support the hypothesis that differences in invasion phenotypes play an important role in malaria virulence and indicate that considerable gaps remain in our knowledge of the molecular basis of invasion pathways in natural P. falciparum infections.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
 
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Created: Fri, 21 Jun 2013, 18:18:31 EST by Susana Nery on behalf of School of Public Health