A dual compartment diffusion chamber for studying axonal chemotaxis in 3D collagen

Pujic, Zac and Goodhill, Geoffrey J. (2013) A dual compartment diffusion chamber for studying axonal chemotaxis in 3D collagen. Journal of Neuroscience Methods, 215 1: 53-59. doi:10.1016/j.jneumeth.2013.02.008


Author Pujic, Zac
Goodhill, Geoffrey J.
Title A dual compartment diffusion chamber for studying axonal chemotaxis in 3D collagen
Journal name Journal of Neuroscience Methods   Check publisher's open access policy
ISSN 0165-0270
1872-678X
Publication date 2013-04
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jneumeth.2013.02.008
Volume 215
Issue 1
Start page 53
End page 59
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Abstract During nervous system development growing axons are often guided by diffusible chemical gradients. An important contribution to our understanding of the mechanisms involved in this process has been made by in vitro assays. However, an inexpensive and simple assay which allows the establishment of stable and reproducible gradients in a 3D collagen environment has been lacking. Here we present a simple two-compartment diffusion chamber for this purpose. We show that gradient steepnesses of up to 2% are achieved within 1. h post setup, and a gradient persists for at least 2 days. We demonstrate the assay by showing robust chemoattraction of dorsal root ganglion neurites by gradients of nerve growth factor (NGF), and chemorepulsion of olfactory bulb neurites by gradients of Slit2.
Keyword Axon guidance
Gradient
Chemotropism
NGF
Slit2
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Queensland Brain Institute Publications
Official 2014 Collection
 
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