Influence of simulated gastrointestinal conditions on particle-induced cytotoxicity and interleukin-8 regulation in differentiated and undifferentiated Caco-2 cells

Gerloff, Kirsten, Pereira, Dora I. A., Faria, Nuno, Boots, Agnes W., Kolling, Julia, Foerster, Irmgard, Albrecht, Catrin, Powell, Jonathan J. and Schins, Roel P. F. (2013) Influence of simulated gastrointestinal conditions on particle-induced cytotoxicity and interleukin-8 regulation in differentiated and undifferentiated Caco-2 cells. Nanotoxicology, 7 4: 353-366. doi:10.3109/17435390.2012.662249


Author Gerloff, Kirsten
Pereira, Dora I. A.
Faria, Nuno
Boots, Agnes W.
Kolling, Julia
Foerster, Irmgard
Albrecht, Catrin
Powell, Jonathan J.
Schins, Roel P. F.
Title Influence of simulated gastrointestinal conditions on particle-induced cytotoxicity and interleukin-8 regulation in differentiated and undifferentiated Caco-2 cells
Journal name Nanotoxicology   Check publisher's open access policy
ISSN 1743-5390
1743-5404
Publication date 2013-06
Year available 2013
Sub-type Article (original research)
DOI 10.3109/17435390.2012.662249
Open Access Status
Volume 7
Issue 4
Start page 353
End page 366
Total pages 14
Place of publication London, United Kingdom
Publisher Informa Healthcare
Collection year 2014
Language eng
Formatted abstract
 Novel aspects of engineered nanoparticles offer many advantages for optimising food products and packaging. However, their potential hazards in the gastrointestinal tract require further investigation. We evaluated the toxic and inflammatory potential of two types of particles that might become increasingly relevant to the food industry, namely SiO2 and ZnO. The materials were characterised for their morphology, oxidant generation and hydrodynamic behaviour. Cytotoxicity and interleukin-8 mRNA and protein expression were evaluated in human intestinal Caco-2 cells. Particle pretreatment under simulated gastric and intestinal pH conditions resulted in reduced acellular ROS formation but did not influence cytotoxicity (WST-1 assay) or IL-8 expression. However, the differentiation status of the cells markedly determined the cytotoxic potency of the particles. Further research is needed to determine the in vivo relevance of our current observations regarding the role of particle aggregation and the stage of intestinal epithelial cell differentiation in determining the hazards of ingested particles.
Keyword SiO2
ZnO
Caco-2
Differentiation
Digestion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
Official 2014 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 27 times in Scopus Article | Citations
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