Shedding light on the Microbial Community of the macropod foregut using 454-Amplicon Pyrosequencing

Gulino, Lisa-Maree, Ouwerkerk, Diane, Kang, Alicia Y. H., Maguire, Anita J., Kienzle, Marco and Klieve, Athol V. (2013) Shedding light on the Microbial Community of the macropod foregut using 454-Amplicon Pyrosequencing. PLoS One, 8 4: e61463.1-e61463.12. doi:10.1371/journal.pone.0061463


Author Gulino, Lisa-Maree
Ouwerkerk, Diane
Kang, Alicia Y. H.
Maguire, Anita J.
Kienzle, Marco
Klieve, Athol V.
Title Shedding light on the Microbial Community of the macropod foregut using 454-Amplicon Pyrosequencing
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-04
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.pone.0061463
Open Access Status DOI
Volume 8
Issue 4
Start page e61463.1
End page e61463.12
Total pages 12
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2014
Language eng
Formatted abstract
Twenty macropods from five locations in Queensland, Australia, grazing on a variety of native pastures were surveyed and the bacterial community of the foregut was examined using 454-amplicon pyrosequencing. Specifically, the V3/V4 region of 16S rRNA gene was examined. A total of 5040 OTUs were identified in the data set (post filtering). Thirty-two OTUs were identified as ‘shared’ OTUS (i.e. present in all samples) belonging to either Firmicutes or Bacteroidetes (Clostridiales/Bacteroidales). These phyla predominated the general microbial community in all macropods. Genera represented within the shared OTUs included: unclassified Ruminococcaceae, unclassified Lachnospiraceae, unclassified Clostridiales, Peptococcus sp. Coprococcus spp., Streptococcus spp., Blautia sp., Ruminoccocus sp., Eubacterium sp., Dorea sp., Oscillospira sp. and Butyrivibrio sp. The composition of the bacterial community of the foregut samples of each the host species (Macropus rufus, Macropus giganteus and Macropus robustus) was significantly different allowing differentiation between the host species based on alpha and beta diversity measures. Specifically, eleven dominant OTUs that separated the three host species were identified and classified as: unclassified Ruminococcaceae, unclassified Bacteroidales, Prevotella spp. and a Syntrophococcus sucromutans. Putative reductive acetogens and fibrolytic bacteria were also identified in samples. Future work will investigate the presence and role of fibrolytics and acetogens in these ecosystems. Ideally, the isolation and characterization of these organisms will be used for enhanced feed efficiency in cattle, methane mitigation and potentially for other industries such as the biofuel industry.
Keyword Ribosomal Rna Genes
Prevotella ruminicola
Bacterial Communities
Phylogenetic Analysis
Fibrolytic Enzymes
Methane Production
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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