Chemical methods for peptide and protein production

Chandrudu, Saranya, Simerska, Pavla and Toth, Istvan (2013) Chemical methods for peptide and protein production. Molecules, 18 4: 4373-4388. doi:10.3390/molecules18044373

Author Chandrudu, Saranya
Simerska, Pavla
Toth, Istvan
Title Chemical methods for peptide and protein production
Journal name Molecules   Check publisher's open access policy
ISSN 1420-3049
Publication date 2013-04-12
Year available 2013
Sub-type Critical review of research, literature review, critical commentary
DOI 10.3390/molecules18044373
Open Access Status DOI
Volume 18
Issue 4
Start page 4373
End page 4388
Total pages 16
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2014
Language eng
Abstract Since the invention of solid phase synthetic methods by Merrifield in 1963, the number of research groups focusing on peptide synthesis has grown exponentially. However, the original step-by-step synthesis had limitations: the purity of the final product decreased with the number of coupling steps. After the development of Boc and Fmoc protecting groups, novel amino acid protecting groups and new techniques were introduced to provide high quality and quantity peptide products. Fragment condensation was a popular method for peptide production in the 1980s, but unfortunately the rate of racemization and reaction difficulties proved less than ideal. Kent and co-workers revolutionized peptide coupling by introducing the chemoselective reaction of unprotected peptides, called native chemical ligation. Subsequently, research has focused on the development of novel ligating techniques including the famous click reaction, ligation of peptide hydrazides, and the recently reported α-ketoacid- hydroxylamine ligations with 5- oxaproline. Several companies have been formed all over the world to prepare high quality Good Manufacturing Practice peptide products on a multi-kilogram scale. This review describes the advances in peptide chemistry including the variety of synthetic peptide methods currently available and the broad application of peptides in medicinal chemistry.
Keyword Peptide
Native chemical ligation
Peptide synthesis
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Non HERDC
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School of Chemistry and Molecular Biosciences
School of Pharmacy Publications
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