Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Wang, Hui, Qian, Zhengjiang, Ma, Sanmei, Zhou, Yuchuan, Patrick, John W., Duan, Xuewu, Jiang, Yueming and Qu, Hongxia (2013) Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.). BMC Plant Biology, 13 . doi:10.1186/1471-2229-13-55

Author Wang, Hui
Qian, Zhengjiang
Ma, Sanmei
Zhou, Yuchuan
Patrick, John W.
Duan, Xuewu
Jiang, Yueming
Qu, Hongxia
Title Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)
Formatted title
Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)
Journal name BMC Plant Biology   Check publisher's open access policy
ISSN 1471-2229
Publication date 2013-04
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1471-2229-13-55
Open Access Status DOI
Volume 13
Total pages 16
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2014
Language eng
Formatted abstract
Background: Recent studies have demonstrated that cellular energy is a key factor switching on ripening and senescence of fruit. However, the factors that influence fruit energy status remain largely unknown.

Results: HPLC profiling showed that ATP abundance increased significantly in developing preharvest litchi fruit and was strongly correlated with fruit fresh weight. In contrast, ATP levels declined significantly during postharvest fruit senescence and were correlated with the decrease in the proportion of edible fruit. The five gene transcripts isolated from the litchi fruit pericarp were highly expressed in vegetative tissues and peaked at 70 days after flowering (DAF) consistent with fruit ADP concentrations, except for uncoupling mitochondrial protein 1 (UCP1), which was predominantly expressed in the root, and ATP synthase beta subunit (AtpB), which was up-regulated significantly before harvest and peaked 2 days after storage. These results indicated that the color-breaker stage at 70 DAF and 2 days after storage may be key turning points in fruit energy metabolism. Transcript abundance of alternative oxidase 1 (AOX1) increased after 2 days of storage to significantly higher levels than those of LcAtpB, and was down-regulated significantly by exogenous ATP. ATP supplementation had no significant effect on transcript abundance of ADP/ATP carrier 1 (AAC1) and slowed the changes in sucrose non-fermenting-1-related kinase 2 (SnRK2) expression, but maintained ATP and energy charge levels, which were correlated with delayed senescence.

Conclusions: Our results suggest that senescence of litchi fruit is closely related with energy. A surge of LcAtpB expression marked the beginning of fruit senescence. The findings may provide a new strategy to extend fruit shelf life by regulating its energy level.
Keyword Controlled atmosphere storage
Cut carnation flowers
Capsicum-Annuum L.
Alternative oxidase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 55.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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