Clonal Relationships among Blood-Stream Isolates of Escherichia coli

Maslow, Joel N., Whittam, Thomas S., Gilks, Charles F., Wilson, Richard A., Mulligan, Maury Ellis, Adams, Kenneth S. and Arbeit, Robert D. (1995) Clonal Relationships among Blood-Stream Isolates of Escherichia coli. Infection and Immunity, 63 7: 2409-2417.

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Author Maslow, Joel N.
Whittam, Thomas S.
Gilks, Charles F.
Wilson, Richard A.
Mulligan, Maury Ellis
Adams, Kenneth S.
Arbeit, Robert D.
Title Clonal Relationships among Blood-Stream Isolates of Escherichia coli
Formatted title
Clonal Relationships among Blood-Stream Isolates of Escherichia coli
Journal name Infection and Immunity
ISSN 1098-5522
Publication date 1995-07
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 63
Issue 7
Start page 2409
End page 2417
Total pages 9
Place of publication Washington, DC 20036 United States
Publisher American Society for Microbiology
Language eng
Abstract The clonal relationships among 187 bloodstream isolates of Escherichia coli from 179 patients at Boston, Mass., Long Beach, Calif., and Nairobi, Kenya, were determined by multilocus enzyme electrophoresis (MLEE), analysis of polymorphisms associated with the ribosomal operon (ribotyping), and serotyping. MLEE based on 20 enzymes resolved 101 electrophoretic types (ETs), forming five clusters; ribotyping resolved 56 distinct patterns concordant with the analysis by MLEE. The isolates at each study site formed a genetically diverse group and demonstrated similar clonal structures, with the same small subset of lineages accounting for the majority of isolates at each site. Moreover, two ribotypes accounted for approximately 30% of the isolates at each study site. One cluster contained the majority (65%) of isolates and, by direct comparison of the ETs and ribotypes of individual isolates, was genetically indistinguishable from the largest cluster for each of two other collections of E. coli causing pyelonephritis and neonatal meningitis (R. K. Selander, T. K. Korhonen, V. Väisänen-Rhen, P. H. Williams, P. E. Pattison, and D. A. Caugent, Infect. Immun. 52:213-222, 1986; M. Arthur, C. E. Johnson, R. H. Rubin, R. D. Arbeit, C. Campanelli, C. Kim, S. Steinbach, M. Agarwal, R. Wilkinson, and R. Goldstein, Infect. Immun. 57:303-313, 1989), thus defining a virulent set of lineages. The isolates within these virulent lineages typically carried DNA homologous to the adhesin operon pap or sfa and the hemolysin operon hly and expressed O1, O2, O4, O6, O18, O25, or O75 antigens. DNA homologous to pap was distributed among isolates of each major cluster, whereas hly was restricted to isolates of two clusters, typically detected in pap-positive strains, and sfa was restricted to isolates of one cluster, typically detected in pap- and hly-positive strains. The occurrence of pap-positive isolates in the same geographically and genetically divergent lineages suggests that this operon was acquired early in the radiation of E. coli, while hly and sfa were acquired subsequently, most likely by pap-positive and pap- and hly-positive precursors, respectively.
Keyword Mannose-Resistant Hemagglutination
Human Extraintestinal Infections
Urinary-Tract Infections
Virulence Factors
Host Factors
Iron Uptake
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
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Citation counts: TR Web of Science Citation Count  Cited 60 times in Thomson Reuters Web of Science Article | Citations
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