Molecular Epidemiology of Penicillin-Resistant Pneumococci Isolated in Nairobi, Kenya

Kell, Christopher M., Jordens, J. Zoe, Daniels, Maggie, Coffey, Tracey J., Bates, Janice, Paul, John, Gilks, Charles and Spratt, Brian G. (1993) Molecular Epidemiology of Penicillin-Resistant Pneumococci Isolated in Nairobi, Kenya. Infection and Immunity, 61 10: 4382-4391.

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Author Kell, Christopher M.
Jordens, J. Zoe
Daniels, Maggie
Coffey, Tracey J.
Bates, Janice
Paul, John
Gilks, Charles
Spratt, Brian G.
Title Molecular Epidemiology of Penicillin-Resistant Pneumococci Isolated in Nairobi, Kenya
Journal name Infection and Immunity
ISSN 1098-5522
1070-6313
Publication date 1993-10
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 61
Issue 10
Start page 4382
End page 4391
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract A total of 26% of the pneumococci isolated from an outpatient clinic in Nairobi, Kenya, during 1991 to 1992 had intermediate levels of penicillin resistance. Gene fingerprinting and DNA sequencing were used to distinguish the penicillin-binding protein (PBP) 1A, 2B, and 2X genes in 23 resistant isolates. Isolates were grouped into those that had identical forms of each of the three PBP genes (fingerprint groups) and those that had identical rRNA gene restriction patterns (ribotypes). Both methods divided the isolates into 11 groups. In a few cases, horizontal gene transfer appeared to have distributed an identical altered PBP gene into different pneumococcal lineages. Eight isolates were indistinguishable by ribotyping or multilocus enzyme electrophoresis and contained identical PBP 1A genes. Although these isolates were therefore members of the same clone, they were divided into two fingerprint groups which contained different PBP 2X and 2B genes. Presumably, members of this clone have acquired different altered PBP 2X and 2B genes on two separate occasions. One of these fingerprint groups contained isolates of serotype 14, whereas the other contained isolates of both serotypes 14 and 7. The identification of isolates in the latter group that are identical by all criteria, except serotype, implies the occurrence of a change in serotype. The predominant serotypes of the penicillin-resistant pneumococci from Nairobi were serotypes 14 and 19. In both cases, isolates of the same serotype which required the same MIC of penicillin were not members of a single clone, indicating that identity of serotype and MIC are not sufficient criteria for defining clones of resistant pneumococci even when the bacteria are isolated from a single clinic.
Keyword Binding Protein Genes
Streptococcus-Pneumoniae
Antimicrobial Resistance
Nucleotide-Sequences
Horizontal Transfer
Dna
Relatedness
Genetics
Spain
2X
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
 
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