DEER-Stitch: combining three- and four-pulse DEER measurements for high sensitivity, deadtime free data

Lovett, J. E., Lovett, B. W. and Harmer, J. (2012) DEER-Stitch: combining three- and four-pulse DEER measurements for high sensitivity, deadtime free data. Journal of Magnetic Resonance, 223 98-106. doi:10.1016/j.jmr.2012.08.011


Author Lovett, J. E.
Lovett, B. W.
Harmer, J.
Title DEER-Stitch: combining three- and four-pulse DEER measurements for high sensitivity, deadtime free data
Journal name Journal of Magnetic Resonance   Check publisher's open access policy
ISSN 1090-7807
1096-0856
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1016/j.jmr.2012.08.011
Volume 223
Start page 98
End page 106
Total pages 9
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
Over approximately the last 15 years the electron paramagnetic resonance (EPR) technique of double electron electron resonance (DEER) has attracted considerable attention since it allows for the precise measurement of the dipole–dipole coupling between radicals and thus can lead to distance information between pairs of radicals separated by up to ca. 8 nm. The “deadtime free” 4-pulse DEER sequence is widely used but can suffer from poor sensitivity if the electron spin-echo decays too quickly to allow collection of a sufficiently long time trace. In this paper we present a method which takes advantage of the much greater sensitivity that the 3-pulse sequence offers over the 4-pulse sequence since the measured electron spin-echo intensity (for equal sequence lengths) is greater. By combining 3- and 4-pulse DEER time traces using a method coined DEER-Stitch (DEERS) accurate dipole–dipole coupling measurements can be made which combine the sensitivity of the 3-pulse DEER sequence with the deadtime free advantage of the 4-pulse DEER sequence. To develop the DEER-Stitch method three systems were measured: a semi-rigid bis-nitroxide labeled nanowire, the bis-nitroxide labeled protein CD55 with a distance between labels of almost 8 nm and a dimeric copper amine oxidase from Arthrobacter globiformis (AGAO).
Keyword Pulsed electron paramagnetic resonance
Deer
Deers
Dipolar coupling
Distance measurements
Nitroxide
Copper protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
 
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